Rat TrkA ELISA Kit

价格： ￥2480

品牌：BOSTER 品牌认证

货号：EK0847

产品详情

产品概况

货号	EK0847
产品名称	Rat TrkA ELISA Kit
产品描述	For quantitative detection of rat TrkA in cell culture supernates and cell lysates.
免疫原	NSO, A33-P418
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	78pg/ml-5000pg/ml
基因名称	NTRK1
基因全名	neurotrophic tyrosine kinase, receptor, type 1
Uniprot ID	P35739
指标别名	High affinity nerve growth factor receptor\|MTC\|Neurotrophic tyrosine kinase receptor type 1\|NTRK1\|NTRK1_HUMAN\|Oncogene TRK\|p140-TrkA\|TRK\|Trk-A\|TRK1\|TRK1-transforming tyrosine kinase protein
背景资料	Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part(A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.
显色时间	20-25分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0847-CAP	96-well plate precoated with anti- rat TrkA antibody	1
EK0847-ST	Lyophilized recombinant rat TrkA standard	10ng/tube×2
EK0847-DA	Biotinylated anti- rat TrkA antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。

2.自动洗板机.

3.移液器和枪头。建议使用多通道移液器/排枪。

4.Eppendorf 管。

5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	51	251	890	60	259	903
Standard deviation	2.24	14.06	59.96	3.54	18.6	70.4
CV(%)	4.4%	5.6%	6.4%	5.9%	7.2%	7.8%