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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma( heparin, EDTA).
规格 ：96T
产品概况
| 货号 | EK0999 |
|---|---|
| 产品名称 | Human ICAM2 ELISA Kit |
| 产品描述 | For quantitative detection of human ICAM-2 in cell culture supernates, serum and plasma( heparin, EDTA). |
| 免疫原 | NSO, K25-Q223 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 312pg/ml-20000pg/ml |
| 基因名称 | ICAM2 |
| 基因全名 | intercellular adhesion molecule 2 |
| Uniprot ID | P13598 |
| 指标别名 | Intercellular adhesion molecule 2|Ly60|CD102 |
| 背景资料 | Intercellular adhesion molecule 2(ICAM2), also known as CD102(Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0999-CAP | 96-well plate precoated with anti- human ICAM-2 antibody | 1 |
| EK0999-ST | Lyophilized recombinant human ICAM-2 standard | 20ng/tube×2 |
| EK0999-DA | Biotinylated anti- human ICAM-2 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 640 | 2403 | 7918 | 592 | 2447 | 7410 |
| Standard deviation | 38.4 | 151.38 | 443.4 | 38.48 | 198.2 | 437.19 |
| CV(%) | 6% | 6.3% | 5.6% | 6.5% | 8.1% | 5.9% |
Human ICAM2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.118 | 0.232 | 0.343 | 0.508 | 0.910 | 1.463 | 1.951 | 2.583 |
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入驻年限：17年
