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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
规格 ：96T
产品概况
| 货号 | EK0945 |
|---|---|
| 产品名称 | Human Endothelin ELISA Kit |
| 产品描述 | For quantitative detection of human Endothelin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | human peptide, C53-W73 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <0.5pg/ml |
| 检测范围 | 3.9pg/ml-250pg/ml |
| 基因名称 | EDN1 |
| 基因全名 | endothelin 1 |
| Uniprot ID | P05305 |
| 指标别名 | Big endothelin 1|ET1|HDLCQ7|PPET1|Preproendothelin 1|EDN2|EDN2_HUMAN|Endothelin 2|Endothelin-2|ET 2|ET-2|ET2|PPET2|Preproendothelin 2|Preproendothelin-2|EDN3|EDN3_HUMAN|Endothelin-3|ET 3|ET-3|PPET3|Preproendothelin 3|Preproendothelin-3 |
| 背景资料 | The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0945-CAP | 96-well plate precoated with anti- human Endothelin antibody | 1 |
| EK0945-ST | Lyophilized recombinant human Endothelin standard | 1ng/tube×2 |
| EK0945-DA | Biotinylated anti- human Endothelin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 11 | 51 | 131 | 11 | 51 | 130 |
| Standard deviation | 0.62 | 2.75 | 8.64 | 0.62 | 2.95 | 10.92 |
| CV(%) | 5.7% | 5.4% | 6.6% | 5.7% | 5.8% | 8.4% |
Human Endothelin ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)| Concentration(pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.019 | 0.047 | 0.085 | 0.178 | 0.410 | 0.879 | 1.813 | 2.570 |
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入驻年限：17年
