MHC四聚体定制服务价格_品牌:Helixgen-丁香通官网

保存条件：4℃避光保存，禁止冷冻

保质期：90-180天

英文名：MHC Tetramer

库存：大量

供应商：广州好芝生物科技有限公司

MHC四聚体定制服务流程

客户提供需要定制的四聚体的Peptide、MHC Restriction → 分析肽段亲和力并评估是否达到合成要求（2-3个工作日）→ 达到合成要求再接受订单进行生产 / 对于亲和力评分较低的肽段，如果客户有明确的文献及实验支持，可以接受定制（但客户需要承担因合成失败所花费用）→ 生产、质检（4-5周）→ 发货

【产品定制流程图示】

【MHC四聚体产品特性】

高灵敏度：可检测血液中低丰度（＜1%）的抗原特异性T细胞

高特异性：通过MHC四聚体技术极大提高了TCR与MHC-抗原多肽复合物结合的特异性

多样化/个性化：可根据客户需求，合成特异的抗原多肽片段，组合成各种T细胞选择性MHC四聚体

定性/定量分析：可结合流式细胞术实现对抗原特异性T细胞群的定性/定量分析

可分选：可通过流式细胞术分选出抗原特异性T细胞群用于下游其他实验或应用

【MHC四聚体产品用途】

适用于全血、外周血单核细胞（PBMCs）、细胞系等样本类型，能精确测定并可结合免疫磁珠分选技术（Immuno-magnetic sorting）高效分选抗原特异性的T细胞。

可应用于病原体免疫和疫苗研发、抗癌疗效评估、过敏监测与脱敏研究、自身免疫研究等领域。

【MHC四聚体产品组成】

T细胞选择性MHC四聚体（T-Select MHC Tetramers）为即用型试剂，预先标记了荧光染料PE或APC。

【参考文献】

1、Shrestha S, Yang K, Wei J, Karmaus PWF, Neale G, Chi H. Tsc1 promotes the differentiation of memory CD8+ T cells via orchestrating the transcriptional and metabolic programs. PNAS. 2014; 111(41):14858-14863.

2、Yan Yang, Chengwen Liu, Weiyi Peng, et al. Antitumor T-cell responses contribute to the effects of dasatinib on c-KIT mutant murine mastocytoma and are potentiated by anti-OX40. BLOOD, 2012; 120(23): 4533-4543.

3、Carl Weidinger, Patrick J. Shaw, Stefan Feske. STIM1 and STIM2‐mediated Ca2+ influx regulates antitumour immunity by CD8+ T cells. EMBO Mol Med (2013) 5, 1311–1321.

4、Caroline Arber, Xiang Feng, HarshalAbhyankar, et al. Survivin-specific T cell receptor targets tumor but not T cells. The Journal of Clinical Investigation, 2015; 125(1):157-168.

5、Julianna Blagih, François Coulombe, Emma E. Vincent, et al. The Energy Sensor AMPK Regulates T Cell Metabolic Adaptation and Effector Responses In Vivo. Immunity, 2015; 42(1): 41-54.

6、Evan F. Lind, Alisha R. Elford, Pamela S. Ohashi. Micro-RNA 155 Is Required for Optimal CD8+ T Cell Responses to Acute Viral and Intracellular Bacterial Challenges. J Immunol 2013; 190:1210-1216.

7、Mandraju R1, Murray S, Forman J, Pasare C. Differential ability of surface and endosomal TLRs to induce CD8 T cell responses in vivo.J Immunol. 2014 May 1;192(9):4303-15

8、Dolina JS1, Braciale TJ, Hahn YS., Liver-primed CD8+ T cells suppress antiviral adaptive immunity through galectin-9-independent T-cell immunoglobulin and mucin 3 engagement of high-mobility group box 1 in mice, Hepatology. 2014 Apr;59(4):1351-65. doi: 10.1002/hep.26938. Epub 2014 Feb 28.

9、Man K, Miasari M, Shi W, Xin A, Henstridge DC, Preston S, Pellegrini M, Belz GT, Smyth GK, Febbraio MA, Nutt SL, Kallies A. The transcription factor IRF4 is essential for TCR af finity-mediated metabolic programming and clonal expansion of T cells. Nature Immunology. 2013 Nov:14(11):1155-65

10、Regulator of fatty acid metabolism, acetyl coenzyme a carboxylase 1, controls T cell immunity, J Immunol. 2014 Apr 1;192(7):3190-9

11、Margaret Gil, Marcin P. Komorowski, MukundSeshadri, Hanna Rokita, A. J Robert McGray, Mateusz Opyrchal, Kunle O. Odunsi and Danuta Kozbor, CXCL12/CXCR4 Blockade by OncolyticVirotherapy Inhibits Ovarian CancerGrowth by Decreasing Immunosuppressionand Targeting Cancer-Initiating Cells, J Immunol2014; 193:5327-5337

12、Dolina, J.S., Sung, S.S., Novobrantseva, T.I., Nguyen, T.M., Hahn, Y.S., Lipidoid Nanoparticles Containing PD-L1 siRNA Delivered In Vivo Enter Kupffer Cells and Enhance NK and CD8(+) T Cell-mediated Hepatic Antiviral Immunity., MolTher Nucleic Acids. 2013, 2:e72. doi: 10.1038, PMID:23423360 PMCID: PMC3586800.

13、Irina Fernandez, Tracy P. Ooi and Krishnendu Roy, Generation of Functional, Antigen-Specific CD8+ Human T Cells fromCord-Blood Stem Cells using Exogenous Notch and Tetramer-TcRSignaling, Stem Cells, 2013 Aug 13. doi: 10.1002/stem.1512

14、Bridle BW, Clouthier D, Zhang L, Pol J, Chen L, Lichty BD, Bramson JL, Wan Y. Oncolytic vesicular stomatitis virus quantitatively and qualitatively improves primary CD8+ T-cell responses to anticancer vaccines. Oncoimmunology. 2013 Aug 1;2(8):e26013. Epub 2013 Aug 27.

15、Zhang L, Bridle BW, Chen L, Pol J, Spaner D, Boudreau JE, Rosen A, Bassett JD, Lichty BD, Bramson JL, Wan Y. Delivery of viral-vectored vaccines by B cells represents a novel strategy to accelerate CD8(+) T-cell recall responses. Blood. 2013 Mar 28;121(13):2432-9.