protein A elisa kit

Product Summary:

For the quantification of Protein A in crude harvest material or the final product of the very monoclonal antibody for therapy.

Precision:

Intra-Assay Precision (Precision within an assay)one known concentration sample was tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)one known concentration sample was tested in separate assays to assess inter-assay precision.

Glycine plasma

	intra-assay precision			inter-assay precision
Sample	1	2	3	1	2	3
n	15	15	15	15	15	15
mean	214.4	99.88	24.18	231.85	96.4	21.22
standard deviation	7.2	0.06	0.41	15.92	1.8	1.89
CV%	3.36%	0.06%	1.70%	6.87%	1.87%	8.90%

Cell culture media

	intra-assay precision			inter-assay precision
Sample	1	2	3	1	2	3
n	15	15	15	15	15	15
mean	209.2	106.8	25.9	220.45	108.6	30.22
standard deviation	4.6	3.4	0.45	10.225	4.3	2.61
CV%	2.20%	3.18%	1.73%	4.64%	4.00%	8.64%

Recovery:

The recovery of Protein A spiked to 2 different levels throughout the range of the assay in various matrices was evaluated.

sample type	average recovery	range %
cell culture media（n=6)	98.90%	88.6-122.06%
glycine plasma（n=6)	82.07%	74.8-97.86%

Linearity:

Cell culture media（n=6)

Glycine plasma（n=6)

Example Data

Well#	Contents	Abs. at 450nm	Mean
1A	0ng/mL	0.128	0.135
2A	0ng/mL	0.142
1B	62.5ng/mL	0.511	0.526
2B	62.5ng/mL	0.541
3A	125pg/mL	0.922	0.958
3B	125pg/mL	0.994
4A	250pg/mL	1.471	1.489
4B	250pg/mL	1.507
5A	500pg/mL	2.238	2.242
5B	500pg/mL	2.246
6A	1000pg/mL	2.749	2.776
6B	1000pg/mL	2.802