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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK0987 |
|---|---|
| 产品名称 | Mouse complement C5a ELISA Kit |
| 产品描述 | For quantitative detection of mouse Complement C5a in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli, N679-R755 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | C5 |
| 基因全名 | complement component 5 |
| Uniprot ID | P06684 |
| 指标别名 | anaphylatoxin C5a analog|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4|C5|CO5_HUMAN|complement C5|Complement C5 alpha chain|Complement component 5|CPAMD4|ECLZB |
| 背景资料 | C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0987-CAP | 96-well plate precoated with anti- mouse Complement C5a antibody | 1 |
| EK0987-ST | Lyophilized recombinant mouse Complement C5a standard | 10ng/tube×2 |
| EK0987-DA | Biotinylated anti- mouse Complement C5a antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 38 | 229 | 499 | 35 | 224 | 532 |
| Standard deviation | 1.78 | 16.94 | 38.92 | 2.1 | 18.81 | 49.47 |
| CV(%) | 4.7% | 7.4% | 7.8% | 6% | 8.4% | 9.3% |
Mouse complement C5a ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.001 | 0.053 | 0.118 | 0.234 | 0.462 | 0.964 | 1.539 | 2.035 |
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