产品详情
文献和实验
相关推荐
库存 :发货周期:现货
供应商 :博士德生物
应用 :cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 :Mouse
规格 :96T
产品概况
货号 | EK0711 |
---|---|
产品名称 | Mouse CD86 ELISA Kit |
产品描述 | For quantitative detection of mouse CD86 in cell culture supernates and serum. |
免疫原 | NSO, V26-G245 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | CD86 |
基因全名 | CD86 molecule |
Uniprot ID | P42082 |
指标别名 | Activation B7 2 antigen|B7 2|B70|B72|BU63|CD28LG2|CD86 antigen (CD28 antigen ligand 2 B7 2 antigen)|CD86 molecule|CTLA 4 counter receptor B7.2|FUN 1|FUN1|T lymphocyte activation antigen CD86 precursor |
背景资料 | Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0711-CAP | 96-well plate precoated with anti- mouse CD86 antibody | 1 |
EK0711-ST | Lyophilized recombinant mouse CD86 standard | 10ng/tube×2 |
EK0711-DA | Biotinylated anti- mouse CD86 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 179 | 772 | 1639 | 165 | 834 | 1677 |
Standard deviation | 11.09 | 47.86 | 86.86 | 12.54 | 62.55 | 95.58 |
CV(%) | 6.2% | 6.2% | 5.3% | 7.6% | 7.5% | 5.7% |
Mouse CD86 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.067 | 0.125 | 0.170 | 0.307 | 0.525 | 0.897 | 1.449 | 2.211 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限:17年