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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
规格 ：96T
产品概况
| 货号 | EK0698 |
|---|---|
| 产品名称 | Human CD27 ELISA Kit |
| 产品描述 | For quantitative detection of human TNFRSF7 in cell culture supernates and serum. |
| 免疫原 | NSO, T21-I192 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 62.5pg/ml-4000pg/ml |
| 基因名称 | CD27 |
| 基因全名 | CD27 molecule |
| Uniprot ID | P26842 |
| 指标别名 | CD 27|Cd27|CD27 antigen|CD27 molecule|CD27_HUMAN|CD27L receptor|LPFS2|MGC20393|OTTHUMP00000238557|S152|T cell activation antigen CD27|T cell antivation antigen S152|T-cell activation antigen CD27|T14|TNFRSF 7|TNFRSF7|TNFSF7|Tp 55|Tp55|Tumor necrosis factor receptor superfamily member 7 |
| 背景资料 | CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0698-CAP | 96-well plate precoated with anti- human TNFRSF7 antibody | 1 |
| EK0698-ST | Lyophilized recombinant human TNFRSF7 standard | 10ng/tubex2 |
| EK0698-DA | Biotinylated anti- human TNFRSF7 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 103 | 595 | 1421 | 108 | 586 | 1478 |
| Standard deviation | 6.79 | 27.37 | 92.36 | 8.2 | 35.74 | 121.19 |
| CV(%) | 6.6% | 4.6% | 6.5% | 7.6% | 6.1% | 8.2% |
Human CD27 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.036 | 0.118 | 0.194 | 0.331 | 0.587 | 1.009 | 1.567 | 2.242 |
武汉博士德生物工程有限公司
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入驻年限：17年
