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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Rat
规格 ：96T
产品概况
| 货号 | EK1295 |
|---|---|
| 产品名称 | Rat Angiopoietin-1 ELISA Kit |
| 产品描述 | For quantitative detection of rat Angiopoietin-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, H16-F498 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | ANGPT1 |
| 基因全名 | angiopoietin 1 |
| Uniprot ID | O35460 |
| 指标别名 | 1110046O21Rik|AGP 1|AGP1|AGPT|ANG 1|ANG1|Angiopoietin1|ANGPT 1|ANGPT1|KIAA0003 |
| 背景资料 | Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. |
| 显色时间 | 20-25 分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1295-CAP | 96-well plate precoated with anti- rat Angiopoietin-1 antibody | 1 |
| EK1295-ST | Lyophilized recombinant rat Angiopoietin-1 standard | 10ng/tube×2 |
| EK1295-DA | Biotinylated anti- rat Angiopoietin-1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 311 | 1285 | 4930 | 287 | 1289 | 5041 |
| Standard deviation | 18.97 | 60.39 | 295.8 | 18.65 | 70.89 | 317.58 |
| CV(%) | 6.1% | 4.7% | 6% | 6.5% | 5.5% | 6.3% |
Rat Angiopoietin-1 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25 分钟)| Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.151 | 0.192 | 0.247 | 0.329 | 0.522 | 0.868 | 1.421 | 2.159 |
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