产品详情
文献和实验
相关推荐
库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK1222 |
|---|---|
| 产品名称 | Mouse CD13 ELISA Kit |
| 产品描述 | For quantitative detection of Mouse CD13 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, K69-S966 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 312pg/ml-20000pg/ml |
| 基因名称 | ANPEP |
| 基因全名 | alanyl (membrane) aminopeptidase |
| Uniprot ID | P97449 |
| 指标别名 | Alanyl (membrane) aminopeptidase|Alanyl aminopeptidase|Alanyl membrane aminopeptidase|Aminopeptidase M|Aminopeptidase N|AMPN_HUMAN|ANPEP|AP M|AP N|AP-M|AP-N|APN|CD 13|CD13|CD13 antigen|gp150|hAPN|Lap 1|Lap1|Microsomal aminopeptidase|Myeloid plasma membrane glycoprotein CD13|p150|PEPN |
| 背景资料 | Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility. |
| 显色时间 | 15-20 分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1222-CAP | 96-well plate precoated with anti- mouse CD13 antibody | 1 |
| EK1222-ST | Lyophilized recombinant mouse CD13 standard | 20ng/tube×2 |
| EK1222-DA | Biotinylated anti- mouse CD13 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 642 | 1721 | 9368 | 660 | 1550 | 9748 |
| Standard deviation | 50.07 | 134.23 | 543.34 | 57.42 | 127.1 | 633.62 |
| CV(%) | 7.8% | 7.8% | 5.8% | 8.7% | 8.2% | 6.5% |
Mouse CD13 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20 分钟)| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.029 | 0.066 | 0.110 | 0.181 | 0.372 | 0.718 | 1.516 | 2.285 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
