保质期 ：36个月
保存条件 ：-20℃~-70℃
英文名 ：Recombinant Human Interleukin 4
库存 ：现货
供应商 ：特宝生物
CAS号 ：无
规格 ：10μg、30μg、100μg
品名 |
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规格 |
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来源 |
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分子量 |
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纯度 |
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内毒素含量 |
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活性 |
- 用细胞增殖的方法进行测定，以人IL-4依赖的细胞系TF-1作为细胞株。比活性约为1×107IU/mg。
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配方 |
- 本品为无菌冻干粉，缓冲体系为PBS, pH7.4。
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复溶 |
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保存 |
- 冻干样品自生产之日起在-20℃到-70℃可以稳定保存36个月。
- 溶解以后，该细胞因子能在2-8℃的无菌条件下保存一个月，或在-20℃到-70℃的人工除霜的冰箱中保存三个月，而无明显活性损失。
- 避免反复冻融。
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Interleukin4 (IL-4), also known as B cell-stimulatory factor-1, is a cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α helix structure. Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Rα and the common γ chain (a shared subunit of the receptors for IL-2,7,9,15, and 21). The type II receptor on nonhematopoietic cells consists of IL-4 Rα and IL13 Rα1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.
仅供实验室使用。
