文献支持MP-007 Minute™ 哺乳动物细胞/组织线粒体分离试剂盒

保存条件 ：-20℃

英文名 ：Minute™ Mitochondrial Isolation Kit for Mammalian Cells and Tissues

规格 ：50 Preps/4 Preps

Minute^TM 哺乳动物细胞/组织线粒体分离试剂盒

目录号：MP-007

哺乳动物细胞/组织线粒体提取试剂盒由优化的缓冲液系统和2.0ml离心管柱组成。可以快速的从哺乳动物细胞/组织中提取线粒体蛋白。专利离心管柱技术使用方便简单，高产，与其他商业试剂盒相比，可以样品处理范围更广泛，可处理5-50million/样品，并且可以得到完整的线粒体。缓冲液系统不含表面活性剂和EDTA，无需匀浆和组织研磨，操作时间<30分钟。

原理：细胞/组织通过Buffer A致敏，然后细胞以Z字形路径通过离心管柱，在这个过程中细胞膜会破裂。进而通过差速离心和密度离心分离出完整的线粒体，整个过程无需匀浆和超高速离心。

提取出的线粒体可以应用于SDS-PAGE，immunoblottings,ELISA,IP,膜蛋白结构分析，2-D，酶活性检测和其他应用

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with MinuteTM Mitochondria Isolation Kit.
A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues respectively.
B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048,a nuclear envelope marker protein. abcam, Cambridge, MA).
C. Densitometry measurement of mitochondrial marker signals.

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简单，快速，性价比高

得率高，可获得更完整的线粒体

处理样品范围更广

缓冲液系统不含表面活性剂和EDTA

1. Gou, R. et al. (2014). Resveratrol suppresses oxidized low-density lipoprotein-induced macrophage apoptosis through inhibition of intracellular reactive oxygen species generation, lox-1 and the p38 MAPK pathway. Cellular Physiology and Biochemistry. 34:603-616. DOI: 10.1159/000363026

2. Hongli Hu, et al (2016).Acetylationof PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. Hepatology.doi:10.1002/hep.28887
3. Ge Gao , Zhipeng Wang , Lingling Lu , Chunli Duan , Xiaomin Wang , Hui Yang（2017）.Morphological analysis of mitochondria for evaluating the toxicity of α-synuclein in transgenic mice and isolated preparations by atomic force microscopy. Biomedicine & Pharmacotherapy . DOI: 10.1016/j.biopha.2017.11.057
4. Zhang,Liwen , Liu,Jian , Zhou,Fangfang , Wang,Weiming , Chen,Nan(2018). PGC-1α ameliorates kidney fibrosis in mice with diabetic kidney disease through an antioxidative mechanism.MOLECULAR MEDICINE REPORTS 17: 4490-4498, 2018 .DOI: 10.3892/mmr.2018.8433
5. Yao Wu, Wei-Nan Gao, Ya-Nan Xue, Li-Chao, Zhang, Juan-Juan Zhang, Sheng-Yao Lu, Xiao-Yu Yan, Hui-Mei Yu, Jing Su, Lian-Kun Sun(2018).SIRT3 aggravates metformin-induced energy stress and apoptosis in ovarian cancer cells.Experimental Cell Research , 2018 doi.org/10.1016/j.yexcr.2018.03.030
6. Luyan Shen, Boyang Sun ,Jiyao Sheng, Sihang Yu ,Yanqing Li, Huadan Xu, Jing Su ,Liankun Sun (2018).PGC1α promotes cisplatin resistance in human ovarian carcinoma cells through upregulation of mitochondrial biogenesis. International Journal of Oncology.doi.org/10.3892/ijo.2018.4401
7. Han, W., Cao, F., Gao, X. J., Wang, H. B., Chen, F., Cai, S. J., ... & Ding, H. Z. ZIC1 acts a tumor suppressor in breast cancer by targeting survivin. International Journal of Oncology.
8. Jie Ouyang etal (2018).SIRT3 inactivation promotes acute kidney injury through elevated acetylation of SOD2 and p53. journal of surgical research.doi.org/10.1016/j.jss.2018.07.019.
9. Kai Zhou1 et al (2018).VDAC2 interacts with PFKP to regulate glucose metabolism and phenotypic reprogramming of glioma stem cells. Cell Death and Disease .DOI 10.1038/s41419-018-1015-x.
10. Xue YN etal (2018).Zinc and p53 disrupt mitochondrial binding of HK2 by phosphorylating VDAC1.Experimental Cell Research.DOI: 10.1016/j.yexcr.2018.12.002.
11. XY Chen et al(2019).Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells.Oxidative Medicine and Cellular Longevity.doi.org/10.1155/2019/9817576.
12. Ma, J., Chen, L., He, X. X., Wang, Y. J., Yu, H. L., He, Z. X., ... & Zhu, X. J. (2019). Functional prediction and characterization of Dip2 gene in mice. Cell biology international.doi.org/10.1002/cbin.11106.
13. Cai, M., He, P., & Fang, D. L. Hypoxia‑induced mitochondrial translocation of DNM1L increases mitochondrial fission and triggers mPTP opening in HCC cells via activation of HK2. Oncology Reports.doi.org/10.3892/or.2019.7213
14. Guo, Z., Song, T., Xue, Z., Liu, P., Zhang, M., Zhang, X., & Zhang, Z. (2019). Using CETSA assay and a mathematical model to reveal dual Bcl-2/Mcl-1 inhibition and on-target mechanism for ABT-199 and S1. European Journal of Pharmaceutical Sciences, 105105.
15. Yue, J., Shen, Y., Liang, L., Cong, L., Guan, X., Li, Z., ... & Xu, W. In situ and ex situ surface‐enhanced Raman spectroscopy (SERS) analysis of cell mitochondria. Journal of Raman Spectroscopy.
16. Duan, C., Kuang, L., Xiang, X., Zhang, J., Zhu, Y., Wu, Y., ... & Li, T. (2020). Activated Drp1-mediated mitochondrial ROS influence the gut microbiome and intestinal barrier after hemorrhagic shock. Aging (Albany NY), 12(2), 1397.
17. Duan, C., Kuang, L., Xiang, X., Zhang, J., Zhu, Y., Wu, Y., ... & Li, T. (2020). Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH-pathways. Cell Death & Disease, 11(4), 1-19.
18. Han, W., Zhang, C., Shi, C. T., Gao, X. J., Zhou, M. H., Shao, Q. X., ... & Yuan, J. L. (2020). Roles of eIF3m in the tumorigenesis of triple negative breast cancer. Cancer Cell International, 20(1), 1-16.
19. Fei, Q., Ma, H., Zou, J., Wang, W., Zhu, L., Deng, H., ... & Wang, N. (2020). Metformin protects against ischaemic myocardial injury by alleviating autophagy-ROS-NLRP3-mediated inflammatory response in macrophages. Journal of Molecular and Cellular Cardiology.
20. Hu, X., Xia, M., Wang, J., Yu, H., Chai, J., Zhang, Z., ... & Sun, L. (2020). Dual PI3K/mTOR inhibitor PKI-402 suppresses the growth of ovarian cancer cells by degradation of Mcl-1 through autophagy. Biomedicine & Pharmacotherapy, 129, 110397.
21. Lu, D., Yang, Z., Xia, Q., Gao, S., Sun, S., Luo, X., ... & Li, X. ACADSB regulates ferroptosis and affects the migration, invasion and proliferation of colorectal cancer cells. Cell Biology International.
22.Huang, J., Xie, P., Dong, Y. et al. Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury. Cell Death Differ (2020). https://doi.org/10.1038/s41418-020-00641-7

23.Deng, X., Lin, N., Fu, J., Xu, L., Luo, H., Jin, Y., ... & Su, J. (2020). The Nrf2/PGC1α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer. Oxidative Medicine and Cellular Longevity, 2020.

24.Wang, Y., Kang, Y., Qi, C., Zhang, T., Zhao, H., Ji, X., ... & Shi, G. (2020). Pentoxifylline enhances antioxidative capability and promotes mitochondrial biogenesis for improving age-related behavioral deficits. Aging, 12.

25.Luo, Q., Wu, X., Zhao, P., Nan, Y., Chang, W., Zhu, X., ... & Liu, Z. (2021). OTUD1 Activates Caspase‐Independent and Caspase‐Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. Advanced Science, 2002874.

26.She, H., Zhu, Y., Deng, H., Kuang, L., Fang, H., Zhang, Z., ... & Li, T. (2021). Protective effects of dexmedetomidine on the vascular endothelial barrier function by inhibiting mitochondrial fission via ER/mitochondria contact. Frontiers in Cell and Developmental Biology, 9.

27.Zhou, J., Zhao, Y., Guo, Y. J., Zhao, Y. S., Liu, H., Ren, J., ... & Ji, E. S. (2021). A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH. Life Sciences, 119403.

28.Yu, S., Yan, X., Tian, R., Xu, L., Zhao, Y., Sun, L., & Su, J. (2021). An Experimentally Induced Mutation in the UBA Domain of p62 Changes the Sensitivity of Cisplatin by Up-Regulating HK2 Localisation on the Mitochondria and Increasing Mitophagy in A2780 Ovarian Cancer Cells. International Journal of Molecular Sciences, 22(8), 3983.

29.Xueyan Wu; Jing Li; Shaopeng Wang; Liping Jiang; Xiance Sun; et al.(2021).2-Undecanone Protects against Fine Particle-Induced Kidney Inflammation via Inducing Mitophagy.Journal of Agricultural and Food Chemistry.https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.1c01305

30. Zhao, L., Liu, D., Ma, W., Gu, H., Wei, X., Luo, W., & Yuan, Z. (2021). Bhlhe40/Sirt1 Axis-Regulated Mitophagy Is Implicated in All-Trans Retinoic Acid-Induced Spina Bifida Aperta. Frontiers in Cell and Developmental Biology, 9, 1025.

31.Feng, S.-T., Wang, Z.-Z., Yuan, Y.-H., Wang, X.-L., Guo, Z.-Y., Hu, J.-H.,Yan, X., Chen, N.-H., Zhang, Y., Inhibition of dynamin-related protein 1 ameliorates the mitochondrial ultrastructure via PINK1 and Parkin in the mice model of Parkinson’s disease, European Journal of Pharmacology, https://doi.org/10.1016/j.ejphar.2021.174262.

32.Wang, Y., Zhang, T., Zhao, H., Qi, C., Ji, X., Yan, H., ... & Shi, G. (2021). Pentoxifylline Enhances Antioxidative Capability and Promotes Mitochondrial Biogenesis in D-Galactose-Induced Aging Mice by Increasing Nrf2 and PGC-1α through the cAMP-CREB Pathway. Oxidative Medicine and Cellular Longevity, 2021.

33.Duan, C., Kuang, L., Hong, C., Xiang, X., Liu, J., Li, Q., ... & Li, T. (2021). Mitochondrial Drp1 recognizes and induces excessive mPTP opening after hypoxia through BAX-PiC and LRRK2-HK2. Cell Death & Disease, 12(11), 1-10.

34.Li, T., Zhao, G., Zhang, T., Zhang, Z., Chen, X., Song, J., ... & Weng, C. (2021). African Swine Fever Virus pE199L Induces Mitochondrial-Dependent Apoptosis. Viruses, 13(11), 2240.

35.Meng, H., Sun, L. K., Su, J., Yan, W. Y., Jin, Y., Luo, X., ... & Wang, H. L. (2021). Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis. Human Cell, 1-20.

36.Li, X., Hou, P., Ma, W., Wang, X., Wang, H., Yu, Z., ... & He, H. (2021). SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy. Cellular & Molecular Immunology, 1-12.

37.Yan, X., Qu, X., Liu, B., Zhao, Y., Xu, L., Yu, S., ... & SU, J. (2021). Autophagy-induced HDAC6 activity during hypoxia regulates mitochondrial energy metabolism through the β-catenin/COUP-TFII axis in hepatocellular carcinoma cells. Frontiers in Oncology, 427.

38.Xiao, L., Hu, Q., Peng, Y., Zheng, K., Zhang, T., Yang, L., ... & Liu, Y. (2021). TRAP1 suppresses oral squamous cell carcinoma progression by reducing oxidative phosphorylation metabolism of Cancer-associated fibroblasts. BMC cancer, 21(1), 1-14.

39.Cheng, M., Yu, H., Kong, Q., Wang, B., Shen, L., Dong, D., & Sun, L. (2022). The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. International Journal of Molecular Sciences, 23(3), 1320.

40.Liu, J., Li, L., Xie, P., Zhao, X., Shi, D., Zhang, Y., ... & Li, T. (2022). Sevoflurane induced neurotoxicity in neonatal mice links to a GSK3β/Drp1-dependent mitochondrial fission and apoptosis. Free Radical Biology and Medicine.

41.Huang, F., Tang, X., Ye, B., Wu, S., & Ding, K. (2022). PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1. Database, 2022.

42.He X-T, Li X, Zhang M, Tian B-M, Sun L-J, Bi C-S, Deng D-K, Zhou H, Qu HL,Wu C, Chen F-M, Role of molybdenum in material immunomodulation and periodontal wound healing:Targeting immunometabolism and mitochondrial function for macrophage modulation, Biomaterials (2022), doi: https://doi.org/10.1016/j.biomaterials.2022.121439.

43.Zhao, M., Wei, H., Li, C., Zhan, R., Liu, C., Gao, J., ... & Zheng, L. (2022). Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy. Nature Communications, 13(1), 1-18.

44.Weikai Hu, Yinan Guo, Xueyan Wang, Tianyu Cui, Chunyun Li, Ruixia Liu, Chenghong Yin.(2022).Angiotensin-(1–7) promotes mitochondrial translocation of human telomerase reverse transcriptase in HUVECs through the TOM20 complex,Archives of Biochemistry and Biophysics,Volume 722,2022

45.Xu, Y., Chen, R., Yan, J. et al. CD137 Signal Mediates Cardiac Ischemia–Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes. J. of Cardiovasc. Trans. Res. (2022). https://doi.org/10.1007/s12265-022-10240-1

46.Li, B., Liu, L. Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury.Lab Invest (2022). https://doi.org/10.1038/s41374-022-00787-7.

47.Wang, X. L., Feng, S. T., Wang, Y. T., Zhang, N. N., Guo, Z. Y., Yan, X., ... & Zhang, Y. (2022). Mangiferin, a natural glucoxilxanthone, inhibits mitochondrial dynamin-related protein 1 and relieves aberrant mitophagic proteins in mice model of Parkinson's disease. Phytomedicine, 154281.

48.Wu, T., Jia, L., Lei, S., Jiang, H., Liu, J., Li, N., ... & Lei, L. (2022). Host HSPD1 Translocation from Mitochondria to the Cytoplasm Induced by Streptococcus suis Serovar 2 Enolase Mediates Apoptosis and Loss of Blood–Brain Barrier Integrity. Cells, 11(13), 2071.

49.An, W., Zheng, M., Ai, Z., & Chen, Y. (2022). Imbalance in ALR ubiquitination accelerates the progression of nonalcoholic steatohepatitis to hepatocellular carcinoma.

50.Min Wang,Bin Zhang,Chenyang Zhang.(2022).Quantitative Crotonylome Analysis Reveals the Mechanism of Shenkang Injection on Diabetic Nephropathy.Hindawi.Oxidative Medicine and Cellular Longevity

51.Wang, Y., Hu, L. F., Liu, N. H., Han, M. M., Zhou, T. J., Xing, L., & Jiang, H. L. (2022). Suppression of mitochondrial heterogeneity via engineered mitochondria for reversion of mitochondrial disease-related phenotypes.
52.Zhu, Y., Lei, L., Wang, X., Chen, L., Li, W., Li, J., ... & Li, X. (2023). The E3 ubiquitin ligase NEDD4-1 protects against acetaminophen-induced liver injury by targeting VDAC1 for degradation. Acta Pharmaceutica Sinica B.

53.Edwards-Hicks, J., Apostolova, P., Buescher, J. M., Maib, H., Stanczak, M. A., Corrado, M., ... & Pearce, E. L. (2023). Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology, 1-15.

54.Yin, M., Dong, J., Sun, C., Liu, X., Liu, Z., Liu, L., ... & Deng, H. (2023). Raddeanin A Enhances Mitochondrial DNA‐cGAS/STING Axis‐Mediated Antitumor Immunity by Targeting Transactive Responsive DNA‐Binding Protein 43. Advanced Science, 2206737.

55.Dou, Y., & Tan, Y. (2023). Presequence protease reverses mitochondria‐specific amyloid‐β‐induced mitophagy to protect mitochondria. The FASEB Journal, 37(5), e22890.

56.Li, C. Q., Wang, H. W., Zheng, M. M., & Chen, Q. W. (2023). Inhibition of DRP1-dependent mitochondrial fission by Mdivi-1 alleviates atherosclerosis through the modulation of M1 polarization. Journal of Translational Medicine, 21(1), 1-17.

57.Zhang, M., Chen, Y., Qiu, Y., Sun, J., He, J., Liu, Z., ... & Liang, J. (2023). PCSK9 promotes hypoxia-induced EC pyroptosis by regulating Smac mitochondrion-cytoplasm translocation in critical limb ischemia. Basic to Translational Science, 8(9), 1060-1077.

58.Duan, C., Liu, R., Kuang, L., Zhang, Z., Hou, D., Zheng, D., ... & Li, T. (2023). Activated Drp1 Initiates the Formation of Endoplasmic Reticulum‐Mitochondrial Contacts via Shrm4‐Mediated Actin Bundling. Advanced Science, 2304885.

59.Wu, K., Huang, C., Zheng, W., Wu, Y., Huang, Q., Lin, M., ... & Liu, L. (2023). Activation of mitophagy improves cognitive dysfunction in diabetic mice with recurrent non-severe hypoglycemia. Molecular and Cellular Endocrinology, 112109.

60.Zhang, G., Wu, S., & Xia, G. (2023). MiR-326 sponges TET2 triggering imbalance of Th17/Treg differentiation to exacerbate pyroptosis of hepatocytes in concanavalin A-induced autoimmune hepatitis. Annals of Hepatology, 101183.

61.Song, J. X., Chen, Q., Xu, S., Gou, Y. J., Guo, Y. J., Jia, C. L., ... & Ji, E. S. (2023). Hydrogen Attenuates Chronic Intermittent Hypoxia-Induced Cardiac Hypertrophy by Regulating Iron Metabolism. Current Issues in Molecular Biology, 45(12), 10193-102