NE-013 Minute™ 核膜蛋白提取试剂盒价格

保存条件：-20℃

英文名：Minute TM Nuclaer Envelope Protein Extraction Kit

规格：50 Preps/4 Preps

Minute^TM 核膜蛋白提取试剂盒

目录号：NE-013

描述

核膜蛋白是一种非常复杂的膜蛋白，由于与细胞核细胞质相连所以非常难以分离和纯化。传统方法提取和纯化需要非常大的样本量，并且过程乏味冗长。Minute核膜蛋白提取试剂盒是第一个可以快速分离天然核膜蛋白的商业试剂盒，无需使用梯度离心，超速离心。专利离心管柱技术简单，快速方便操作。起始样品量仅需10-20million个细胞，缓冲液不含表面活性剂和EDTA，操作时间<45分钟，并可获得10-50ug蛋白/样品。

应用

提取的蛋白质样品可以应用于SDS-PAGE，immunoblottings，ELISA，IP，蛋白质运输分析，酶活性检测和其他应用。

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Protocol

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产品特点

简单，快速，性价比高

真正未变性的膜蛋白-无表面活性剂及EDTA

小样品量

参考文献

1. Frias A. et al. (2016). A Switch in Akt Isoforms Is Required for Notch-Induced Snail1 Expression and Protection from Cell Death. Mol Cell Biol. 2016 Mar 15; 36(6): 913–922. Prepublished online 2015 Dec 28. Published online 2016 Mar 1. doi: 10.1128/MCB.00842-15
2. Selby, T. L., Biel, N., Varn, M., Patel, S., Patel, A., Hilding, L., ... & Lowrey, A. J. (2019). The Epstein-Barr Virus Oncoprotein, LMP1, Regulates the Function of SENP2, a SUMO-protease. Scientific Reports, 9(1), 9523.doi.org/10.1038/s41598-019-45825-5

3.Shao, Y., Li, L., Liu, L., Yang, Y., Huang, J., Ji, D., ... & Sun, B. (2022). CD44/ERM/F‐actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis. Journal of Cellular and Molecular Medicine.

4.Zervopoulos, S. D., Boukouris, A. E., Saleme, B., Haromy, A., Tejay, S., Sutendra, G., & Michelakis, E. D. (2022). MFN2-driven mitochondria-to-nucleus tethering allows a non-canonical nuclear entry pathway of the mitochondrial pyruvate dehydrogenase complex. Molecular Cell, 82(5), 1066-1077

5.Kovacs, M. T., Vallette, M., Wiertsema, P., Dingli, F., Loew, D., de Freitas Nader, G. P., ... & Ceccaldi, R. (2023). DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C. Molecular Cell.

6.Zhou, X. Y., Lin, B., Chen, W., Cao, R. Q., Guo, Y., Said, A., ... & Zhu, Y. M. (2023). The brain protection of MLKL inhibitor necrosulfonamide against focal ischemia/reperfusion injury associating with blocking the nucleus and nuclear envelope translocation of MLKL and RIP3K. Frontiers in Pharmacology, 14.

该产品被引用文献

1. Frias A. et al. (2016). A Switch in Akt Isoforms Is Required for Notch-Induced Snail1 Expression and Protection from Cell Death. Mol Cell Biol. 2016 Mar 15; 36(6): 913–922. Prepublished online 2015 Dec 28. Published online 2016 Mar 1. doi: 10.1128/MCB.00842-15
2. Selby, T. L., Biel, N., Varn, M., Patel, S., Patel, A., Hilding, L., ... & Lowrey, A. J. (2019). The Epstein-Barr Virus Oncoprotein, LMP1, Regulates the Function of SENP2, a SUMO-protease. Scientific Reports, 9(1), 9523.doi.org/10.1038/s41598-019-45825-5

3.Shao, Y., Li, L., Liu, L., Yang, Y., Huang, J., Ji, D., ... & Sun, B. (2022). CD44/ERM/F‐actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis. Journal of Cellular and Molecular Medicine.

4.Zervopoulos, S. D., Boukouris, A. E., Saleme, B., Haromy, A., Tejay, S., Sutendra, G., & Michelakis, E. D. (2022). MFN2-driven mitochondria-to-nucleus tethering allows a non-canonical nuclear entry pathway of the mitochondrial pyruvate dehydrogenase complex. Molecular Cell, 82(5), 1066-1077

5.Kovacs, M. T., Vallette, M., Wiertsema, P., Dingli, F., Loew, D., de Freitas Nader, G. P., ... & Ceccaldi, R. (2023). DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C. Molecular Cell.

6.Zhou, X. Y., Lin, B., Chen, W., Cao, R. Q., Guo, Y., Said, A., ... & Zhu, Y. M. (2023). The brain protection of MLKL inhibitor necrosulfonamide against focal ischemia/reperfusion injury associating with blocking the nucleus and nuclear envelope translocation of MLKL and RIP3K. Frontiers in Pharmacology, 14.