HD-021 Minute™ 总蛋白提取试剂盒(角质化组织)价格

保存条件：RT

英文名：MinuteTM Protein Extraction Kit for Hair and Nails

规格：20 Preps

说明：动物角质化组织中（包括但不仅限于毛发，指甲，角，羊毛）蛋白质含量占80% 。这些组织中含有很多分子量10-135KD不同类型的α-角蛋白。蛋白质大多数分子量为10-65KD。传统方法从角质化组织中提取蛋白质，其缓冲液中通常含有高浓度的尿素，硫niao，盐酸胍等强变性剂，虽然这些变性剂是比较有效的，但是由于缓冲液与组织比值过大，提取出的蛋白质浓度通常很低，进一步分析前还需浓缩，高浓度变性剂很可能干扰下游实验。HD-021试剂盒无需使用上面提到的强变性剂，可有效的提取动物角化组织中的蛋白质，提取出的蛋白质无需浓缩，可直接用于SDS-PAGE。缓冲液中含有0.5%SDS和其他化学成分。蛋白得率可达1-2mg/ml.
特点：

高得率，可直接用于下游实验
不含高浓度强变性剂
储存条件：储存于室温

参考文献：
1.Lee, S. H., Baldina, E., Lee, E., & Youm, Y. (2021). Social connectedness and hair cortisol in community-dwelling older adults. Comprehensive Psychoneuroendocrinology, 100053.

2.Marsden, A. J., Riley, D. R., Birkett, S., Rodriguez-Barucg, Q., Guinn, B. A., Carroll, S., ... & Beltran-Alvarez, P. (2021). Love is in the hair: arginine methylation of human hair proteins as novel cardiovascular biomarkers. Amino Acids, 1-10.

3.Kim, W. J., Park, K. M., Park, J. T., Seo, E., An, S. K., Park, H. Y., & Lee, E. (2021). Sex-specific association of hair cortisol concentration with stress-related psychological factors in healthy young adults. Biology of Sex Differences, 12(1), 1-7.

4.Kim, W. J., Park, K. M., Park, J. T., Seo, E., Bang, M., An, S. K., ... & Lee, E. (2021). Effect of Childhood Trauma on the Association Between Stress-Related Psychological Factors and Hair Cortisol Level in Young Adults. Psychiatry Investigation, 18(11), 1131.

5.Lee, S., Park, J. T., Bang, M., An, S. K., Namkoong, K., Park, H. Y., & Lee, E. (2022). Theory of mind and hair cortisol in healthy young adults: the moderating effects of childhood trauma. European Journal of Psychotraumatology, 13(2), 2116826.

6.Fuse N, Morita S,Matsue Y.(2023). Age-related hair denaturation related to protein carbonyls. Int J Cosmet Sci. 2023;00:1–9.

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该产品被引用文献

1.Lee, S. H., Baldina, E., Lee, E., & Youm, Y. (2021). Social connectedness and hair cortisol in community-dwelling older adults. Comprehensive Psychoneuroendocrinology, 100053.

2.Marsden, A. J., Riley, D. R., Birkett, S., Rodriguez-Barucg, Q., Guinn, B. A., Carroll, S., ... & Beltran-Alvarez, P. (2021). Love is in the hair: arginine methylation of human hair proteins as novel cardiovascular biomarkers. Amino Acids, 1-10.

3.Kim, W. J., Park, K. M., Park, J. T., Seo, E., An, S. K., Park, H. Y., & Lee, E. (2021). Sex-specific association of hair cortisol concentration with stress-related psychological factors in healthy young adults. Biology of Sex Differences, 12(1), 1-7.

4.Kim, W. J., Park, K. M., Park, J. T., Seo, E., Bang, M., An, S. K., ... & Lee, E. (2021). Effect of Childhood Trauma on the Association Between Stress-Related Psychological Factors and Hair Cortisol Level in Young Adults. Psychiatry Investigation, 18(11), 1131.

5.Lee, S., Park, J. T., Bang, M., An, S. K., Namkoong, K., Park, H. Y., & Lee, E. (2022). Theory of mind and hair cortisol in healthy young adults: the moderating effects of childhood trauma. European Journal of Psychotraumatology, 13(2), 2116826.

6.Fuse N, Morita S,Matsue Y.(2023). Age-related hair denaturation related to protein carbonyls. Int J Cosmet Sci. 2023;00:1–9.