TEMED (Sigma代理)T7024-25ml

包装

25, 50, 100 mL in glass bottle

Quantity

针对 50mL 丙烯酰胺凝胶溶液，建议用量为 35μL。

Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.¹,²,³
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.⁴ TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.⁵ Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.⁶
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.⁷ TEMED has been used in the HPLC of small organic molecules.⁸