英文名 ：通用型SYBR荧光定量

库存 ：SYBR® Fast通用型

供应商 ：研卉生物

CAS号 ：SYBR® Fast通用型

规格 ：kit of 10 mL (1000 x 20 μL rxn

通用型SYBR快速定量 KAPA KK4601
SYBR® Fast通用型

1)高的反应效率使循环阀值早出现，并且灵敏度极高；

2）高的荧光强度提高了信噪比；

3）宽动态范围和很好的线性；

4）反应快速，节约40~60%的反应时间；

5）快速/标准反应程序结果无差异。

　　客户评语摘录：

　　“I tried countless combinations of qPCR reagents, buffers and primer sets before contacting KAPA Biosystems for a sample of their KAPA SYBR® FAST qPCR Kit (universal), somewhat in desperation. I had immediate success and have never looked back. I trust the ability of the enzyme to generate reliable results quick and simply, typically without optimization. I refuse to use other reagents now”

　　Dr. phillippa Taberlay, Ph.D Senior Research Officer Epigenetics Research Group.

　　KAPA公司针对市场上不同型号的qPCR仪开发了相应的试剂，详细见下表。

　　1、应用范围

　　1 )基因表达分析;2 )生物芯片验证;3 )低拷贝基因验证;4 )基因敲除验证。

　　2、技术优点

　　1 )高的反应效率使达到阈值的循环数早出现，并且灵敏度极高;2)高的荧光强度提高了信噪比;

　　3)宽动态范围和很好的线性; 4)反应快速，减少40-60%的反应时间;5)快速/标准反应程序结果无差异。

　　GC含量、长度各异的10个看家基因，GC含量44.2-62.5%(左图)，长度范围86-249bp(右图)扩增效率无明显差异。扩增试剂：KAPA SYBR® FAST Universal。扩增效率均坐落在95-105%有效区间内。

　　3、同类产品比较

　　4、快速/标准反应程序，结果无差异

　　5、定量反应

　　1)推荐反应体系

　　2)推荐反应参数

　　①Roche仪器反应程序:

　　②非Roche仪器反应程序:

　　6、问题及解决方法

Evolved to shine.

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.

The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.

Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

For Research Use Only. Not for use in diagnostic procedures.

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