病毒包装服务/病毒包装检测/病毒载体构建

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服务名称：病毒包装服务/病毒包装检测/病毒载体

提供商：OBiO和元生物

规格：1.0E+8~1.0E+12

和元生物提供腺相关病毒（AAV）、慢病毒（LV）、腺病毒(ADV)、逆转录病毒（RV）包装服务。这几种病毒的生产和质控采取了国际学术界公认的标准流程，所获得的病毒完全可以满足各类实验的使用要求：

腺相关病毒AAV（和元）的滴度在10¹²~10¹³v.g./ml
慢病毒（和元）的滴度在10⁸~10⁹TU/ml
腺病毒（和元）的滴度在10¹⁰~10¹¹pfu/ml
逆转录病毒（和元）滴度在10⁷~10⁸TU/ml

和元生物的技术团队拥有十年以上的病毒载体开发和病毒包装服务经验。我们依靠坚持不懈的努力和精益求精的态度，推动了病毒载体在国内的技术转化和应用推广。

随着生物医学的飞速发展和基因治疗的日益进步，病毒载体越来越多应用于机制研究和临床前实验。本着服务于客户、服务于科研的宗旨，和元上海将病毒包装服务的稳定和质量作为公司首要目标。在材料质量、操作水平、质量控制等方面都建立了严格的内部标准，力争为客户提供高质量病毒和最准确的结果！

一、病毒载体服务介绍

病毒载体是当前生物医学研究中重要的基因转导工具。一方面病毒载体经过改造具有更强的使用安全性和更快的分子克隆速度；另一方面，病毒的感染能力得到了定向的人工进化：治疗靶点筛选者希望得到更快捷，更广谱的转导工具，而基因治疗却需要更安全更特异的感染特性。在不断涌现的新工具新方法面前，研究者常常会无所适从，既难舍对已有系统的信赖与熟悉，又倾心于新工具的蓬勃前景。

目前生物医学研究中常用的病毒载体包括腺相关病毒、慢病毒、腺病毒、逆转录病毒。表1简单的总结了四种病毒载体的基本生物学特性。和元慢病毒感染范围广，表达稳定长效，已经成为分子生物操作中的常规载体，越来越多的分子生物学技术与方法借助于慢病毒载体得以应用。腺病毒载体作为研究和使用时间最长的载体，虽然存在一些不足，但其特点依然鲜明，即感染速度快，基因装载量大。逆转录载体因其特殊的感染特性，仍在干细胞和血液细胞等分裂细胞研究中占有一席之地。而势头强劲的腺相关病毒因其极高的滴度和低转基因安全性，成为基因治疗研究的新宠儿。

表1 不同病毒的生物学特性比较

病毒特性	病毒类型
病毒特性	慢病毒(LV)	逆转录病毒(RV)	腺相关病毒(AAV)	腺病毒(ADV)
包膜	有	有	无	有
颗粒直径	90-100 nm	90-100 nm	20-30 nm	60-90nm
基因组	dsRNA	dsRNA	ssDNA	dsDNA
表达起始时间	48-72h	48-72h	72-96h	24-48h
表达持续时间	> 2 months	> 2 months	> 6 months	< 1 month
整合方式	随机高频整合	随机高频整合	定向低频整合	非整合

1．腺相关病毒
和元腺相关病毒（adeno-associated virus，AAV）是一类细小病毒，基因组为单链DNA，对分裂细胞和非分裂细胞均具有感染能力。 AAV的血清型是指目前已发现的不同衣壳蛋白的数十种AAV在体内产生不同的抗血清类型，它们对相同的组织和细胞具有不同的感染效率。
重组腺相关病毒rAAV是利用AAV2型基因组与不同的衣壳蛋白结合产生的混合体病毒载体，一般标记为rAAV2/N (N为不同的衣壳血清型)。纽恩生物通过长期的研发，构建了一系列用于基因表达和基因干扰操作的载体。

表2 不同血清型AAV的组织嗜亲性

血清型	适用组织	胞外吸附分子
AAV1	肌肉,心脏,神经	未知
AAV2	体外,神经	硫酸乙酰肝素蛋白多糖
AAV5	肺,眼睛,神经	N-唾液酸
AAV6	肺,心脏	未知
AAV7	肌肉,肝脏	未知
AAV8	肝脏,肌肉,眼睛	未知
AAV9	肺,肝脏，肌肉，心脏	未知
rh10	肋膜,神经	未知

病毒感染实例如下：

2．慢病毒
慢病毒(Lentivirus)：是一类改造自人免疫缺陷病毒(HIV)的病毒载体。它是一种RNA病毒，可利用逆转录酶将外源基因整合到基因组中实现稳定长时表达。目前广泛用于哺乳动物细胞和整体的基因转化操作，具有能同时感染分裂期与非分裂期的细胞等特性。

病毒感染实例如下：

3. 逆转录病毒
逆转录病毒(Retrovirus) ：改自鼠白血病病毒(MMLV),是一种RNA病毒，可以利用逆转录酶将外源基因整合到基因组中实现稳定长时表达。逆转录病毒特异性地感染分裂细胞，对造血干细胞和神经干细胞在体外和体内感染具有良好的效果。

病毒感染实例如下：

4. 腺病毒
腺病毒 (Adenovirus) ：是一种DNA病毒，具有广泛的细胞和组织感染能力。感染过程剧烈，适于在短时间内进行基因高表达的实验。与其它病毒载体相比，腺病毒最大优势在于插入片段较长，表达活性强；可进行扩增操作。

病毒感染实例如下：

二、客户部分发表文章列表

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