ExiPrep™ 16 核酸提取仪

ExiPrep™ 16 Plus是BIONEER公司研发生产的小型全自动核酸提取仪，全血、动植物组织和细胞t等多种样本中提取核酸及纯化，还可以用于重组蛋白的纯化，是一款体积紧凑功能强大的仪器。ExiPrep™16 Plus一次最多可以从16个样本中提取核酸及蛋白纯化，优化好的预安装程序保证实验结果具有良好的重复性。特别是ExiPrep™ 16 Plus中安装了防止移液移动过程产生交叉污染的装置，大大的提高了实验的准确性及结果的可信性。 Patent registration KR 10-1025135, US 2011-0009608 Patent application KR 10-2010-0002021

主要应用

分类	适用领域
大学、研究所及企业研究机构	从多种样本中提取核酸及蛋白质纯化
医院、医学研究所及CDC	需要从血液、组织及多种样本中提取核酸进行检测分析
食品公司及食品安全检测机构	短时间内从多种食材中提取核酸进行GMO/食物中毒检测
军队和法医检测机构	提取核酸进行生物恐怖病原菌及食品中毒病原菌检测

可提取样本

血清、全血、动物组织、植物、培养细胞、CSF、尿液、粪便、裂解细胞等

提取产物类型

DNA -- 基因组DNA、病毒DNA、质粒DNA及DNA片段
RNA -- 总RNA、病毒RNA
蛋白质-- His-tagged 蛋白

产品特点及优势

预安装多种应用程序 ExiPrep™16 Plus 内预安装了超过50种经过优化验证的应用程序，操作者无需编程，只要选择相应程序仪器即可全自动的完成整个实验过程。
Magnetic block & Heating block 为了提高核酸提取及蛋白纯化的效率ExiPrep™16 Plus内置了磁铁和加热模块。在4个位置装有磁铁，使磁珠在各个孔内均一地、快速吸附，可以使核酸提取及蛋白纯化纯度更高效率更快。内置的加热模块能缩短样本裂解所需时间，并且通过加热反应使酒精蒸发完全。(专利申请中).
防交叉污染装置 ExiPrep™ 16 Plus上防污染装置置于移液管下部，主要作用是有效防止核酸提取/纯化和蛋白质纯化过程中产生交叉污染。
便捷的触摸屏操作 使用3.5存触摸屏可以选择实验程序及控制紫外灯给内部杀菌等，还可以实时显示实验进程，操作极为方便。

实验过程

实验数据

1.在细胞中提取基因组DNA (Hela细胞系)

• 样品量: 1X10⁶ cells
• 程序编号: 107
• 仪器运行时间: < 1hr.
• 平均产量: 4-8μg

• 琼脂糖凝胶电泳分析

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).

2.从细菌中提取基因组DNA (E.coli)

• 样本量 1X10⁶ cells
• 程序编号: 109
• 仪器运行时间: <1 hr
• 平均产量: 4-8ug
• 平均产量: 8-12ug

• 凝胶电泳分析

cells of E.coli cells and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

3.从组织中提取基因组DNA (牛肌肉组织)

• 样本量: 1X10⁶ cells
• 程序编号: 109
• 仪器运行时间: <1 hr
• 平均产量: 8-12ug

• 凝胶电泳分析

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 20mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

4.从植物组织中提取基因组DNA (菠菜叶)

• 样本量: 100mg
• 程序编号: 104
• 仪器运行时间: <1 hr
• 平均产量: 3-5ug

• 凝胶电泳分析

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100mg of spinach leaves and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

5. 从培养细胞中提取RNA (HeLa).

• 样本量: 1.0 X 10⁶ cells
• 程序编号: 207
• 平均产量：10 ug
• Average RQS: 9.5 以上

• LabChip GX毛细管电泳分析

Results of capillary electrophoresis of total RNA extracted from HeLa cell(1X106 cells) using LabChip GX(Caliper life science, USA). Average RQS(RNA Quality Score) were at least 9.5t shown).

• Real-time RT-PCR分析总RNA提取效果

Real-time RT-PCR results using an AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer)와 AccuPower®Dualstar™qPCR PreMix(K-6110, Bioneer) and run on Exicycler™96 Real-Time PCR System (A-2060, Bioneer) from total RNA extracted from HeLa cell(1X10⁶ cells)

6. 蛋白质纯化

• SDS-PAGE分析

Fig. 1 Electrophoresis was performed on a vertical gel containing 13% poly-acrylamide.
Lane 1: crude extract of gram negative cell by sonication
Lane 2-3: unbound protein (duplication)
Lane 4, 5, 6, 7, 8, 9, 10, 11: purified PTPase #32S

• ample type : Recombinant E.coli cell
• Starting volume : 500 μL
• Elution volume : 250 μL
• Average yield : 200 μg/well
• Average purity : 80%

Electrophoresis was performed on a vertical gel containing 10% poly-acrylamide.
Lane 1: PTPase #1 (65kD), Lane 2: Polymerase (95kD)
Lane 3: PTPase #11 (36kD), Lane 4: PTPase #37 (18kD)
Lane 5: PTPase #32 (23kD), Lane 6: PTPase #39 (19kD)
purified diverse size of proteins by ExiPrep™ His-tagged protein purification kit using ExiPrepTM16 Plus.

• ample type : Recombinant E.coli cell
• Starting volume : 500 μL
• Elution volume : 250 μL
• Average yield : 200 μg/well
• Average purity : 80%
• Cat. No.	Kits for ExiPrep™ 16	Price
  A-5030	ExiPrep™16 Plus	询价

  
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  Cat. No.	Accessories	Price
  A-5010-A	Accessories Set for ExiPrep™ 16 Plus	询价
  A-5010-A1	Setup Tray	询价
  A-5010-A10	2D-barcode Tube Rack	询价
  A-5010-A2	Disposable Tip Rack	询价
  A-5010-A3	Elution Tube Rack	询价
  A-5010-A4	Reaction Tube Rack	询价
  A-5010-A5	Waste Tray	询价
  A-5010-A6	Hole Puncher (6-hole)	询价
  A-5010-A7	Hole Puncher (12-hole)	询价
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  A-5010-A9	AC Adapter for ExiPrep™16 Plus	询价