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英文名 :Phos-tag™ Acrylamide AAL-107 5mM Aqueous Solution
保存条件 :冷藏
规格 :0.3mL
0.3 mL Package size
Phos-tag™ Acrylamide is a functional molecule that binds specifically phosphorylated ions.
It is applicable for the specific separation of phosphorylated proteins.
Phos-tag™ is a novel phosphate-binding tag at neutral pH* (physiological pH) and was developed by Department of Functional Molecular Science at Hiroshima University.
Wako Distributes specific electrophoretic procedure for the simultaneous analysis of a phosphoprotein isoform and its non-phosphorylated counterpart Manganese (II)-Phos-tag™ SDS-PAGE.
(*: It is in case of Phos-tag™-Zn2+. On the other hand, the stable pH is around 9 in case of Phos-tag™-Mn2+.)
Features:
- Recognition of all phosphorylated forms of Tyr/ Ser / Thr
- Simultaneous detection of phosphorylated / non-phosphorylated proteins without against the phosphorylated protein.
- Simply add Phos-tag™ Acrylamide and MnCl2 solution*2 to acrylamide solution in the preparation of SDS-PAGE gel.
(*: Add 5.0 mM Phos-tag&tade Acrylamide Aqueous Solution and 10 mM MnCl2 solution in equal amount in the gel since one Phos-tag™ binds to two manganese ions (Mn2+).
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入驻年限:11年