LEAF™ Purified anti-human IL-5价格_品牌:Biolegend-丁香通官网

保存条件：蓝冰

克隆性：多克隆

规格：500 ug

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品牌介绍：Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
LEAF™ Purified anti-human IL-5参数介绍>>>>

Clone	JES1-39D10
Isotype	Rat IgG2a, κ
Reactivity	Human, *Cross-Reactivity^:** Cynomolgus, Rhesus
Immunogen	COS-expressed, recombinant human IL-5
Formulation	0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation	The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration	1mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISPOT capture antibody, a concentration range of 4-8 µg/ml is recommended. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-5 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	ELISA Capture - Quality tested ELISPOT Capture, Neut, ICFC, IHC, WB - Reported in the literature
Application Notes	ELISA^1-3 or ELISPOT^4-6 Capture: The Purified JES1-39D10 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the biotinylated JES1-5A10 (Cat. No. 501002/501005) antibody as the detecting antibody. The LEAF™ Purified antibody is suggested for ELISPOT capture. Flow Cytometry⁷: The fluorochrome-labelled JES1-39D10 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization¹: The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500907). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining^8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 to 430406) are specially developed and recommended.
Application References	1. Abrams, J., et al. 1992. Immunol. Rev. 127:5. 2. Butterfield, J., et al. 1992. Blood 79:688. 3. Abrams, J., et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker, New York. p.133. 4. Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.6. 5. Mahanty, S., et al. 1992. J. Immunol. 148:3567. 6. Limaye, A., et al. 1991. J. Clin. Inv. 88:1418. 7. Prussin, C., et al. 1995. J. Immunol. Meth. 188:117. 8. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. 9. Andersson, J., et al. 1994. Immunology 83:16.
Description	IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. The JES1-39D10 antibody can neutralize the bioactivity of natural or recombinant IL-5.
Storage	The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.