PE/Cy7 anti-mouse CD127 (IL-7Rα)

PE/Cy7 anti-mouse CD127 (IL-7Rα)

价格: ¥1365

品牌:Biolegend

货号:135013

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保存条件 :蓝冰

克隆性 :多克隆

规格 :25 ug

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品牌介绍:Biolegend公司自2002年成立以来,一直致力于为生物医药研究领域提供一流的,高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士,建立了一支雄厚技术背景的专业队伍,并通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。短短几年时间,Biolegend公司得到了飞速发展,已跻身全球最先进的抗体公司行列,并在2009年成为美国哈佛大家的独家抗体供应商!Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂,用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中,Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比,为广大科研工作者所接受!同时,公司配备了一支经验丰富的技术服务队伍,为客户提供完美的配色方案及快速的售后处理服务!
PE/Cy7 anti-mouse CD127 (IL-7Rα)参数介绍>>>>
Clone A7R34
Isotype Rat IgG2a, κ
Reactivity Mouse
Immunogen IL-7Ra-IgG1 fusion protein
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration 0.2mg/ml
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications FC - Quality tested
Application Notes

In process development has shown A7R34 is able to block clone SB/199 binding to IL-7R.

Application References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Hashi H, et al. 2001. J. Immunol. 166:3702.
3. Taylor R, et al. 2007. J. Immunol. 178:5659.
4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed

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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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