LEAF™ Purified anti-mouse CD49d价格_品牌:Biolegend-丁香通官网

保存条件：蓝冰

克隆性：多克隆

规格：500 ug

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品牌介绍：Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
LEAF™ Purified anti-mouse CD49d参数介绍>>>>

Clone	R1-2
Isotype	Rat IgG2b, κ
Reactivity	Mouse
Immunogen	AKR/Cum mouse spontaneous T lymphoma line TK1
Formulation	0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation	The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration	1mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	FC - Quality tested IP, Block, IHC - Reported in the literature
Application Notes	Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation^1-4, immunoprecipitation¹, and immunohistochemistry⁵ of frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103610).
Application References	1. Holzmann B, et al. 1989. Cell 56:37. (IP, Block) 2. Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block) 3. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) 4. Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block) 5. Jaspers M, et al. 1995. Differentiation 59:79. (IHC) 6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
Description	CD49d is a 150 kD glycoprotein, also known as α₄ integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α₄β₁) or LPAM-1 (integrin α₄β₇) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10(MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4.
Storage	The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.