脑缺血MCAO模型／MCAO栓线／MCAO线栓／硅胶栓线／硅胶线栓／尼龙栓线／尼龙线栓价格

库存：大量

供应商：北京西浓科技有限公司

现货状态：现货

MCAO栓线简介
国内最早实行标准化、规模化、商品化的MCAO栓线（以国内外发表论文数量为证，2014年前由北京沙东生物公司经营，此后转交北京西浓科技公司）。MCAO栓线（MCAO Monofilaments）是制备大鼠/小鼠（或其它实验动物）局部脑缺血模型的非常关键的实验材料，本产品是用柔韧适度的单丝尼龙线，经显微操作烧制而成，其头端光滑、大小一致，易进入颅内又不至于刺破血管，使用本产品可大大提高模型制备的成功率，及脑缺血范围的稳定性。根据包被材料的不同分为三大类：硅胶包被、多聚赖氨酸包被、无包被。本产品已被美国、加拿大、香港、中国大陆等的科研机构、大学、医院广泛采用。研究论文发表于本专业顶级的期刊：J Neuroscience，Stroke，Journal of Cerebral Blood，Flow & Metabolism，Journal of Neurochemistry等（请下载产品资料）。
各类产品的优缺点：
硅胶栓线：梗死面积相对稳定，但插线操作难度大、死亡率高、不易长时间观察。
无包被的尼龙栓线：插线操作难度小，死亡率相对较低，但梗死面积的一致性略低。
赖氨酸包被的尼龙栓线：插线操作难度小，死亡率相对较低，梗死面积的稳定性介于上述两者之间。

产品分五个级别，每个级别分若干型号和规格。（见MCAO栓线型号/价格表）
A1级：头端烧熔为半球形（下载资料1，2），无包被、无标记、未消毒，需要用户自己消毒、做标记；
A2级：头端烧熔为半球形，无包被、有标记*、已消毒，可以直接使用；
A3级：头端烧熔为半球形，前端包被多聚赖氨酸^#、无标记、已消毒，需要用户自己做标记；
A4级：头端烧熔为半球形，前端包被多聚赖氨酸、有标记、已消毒，即买即用型，不需任何处理。
A5级：头端烧熔为半球形，且前端5-6mm均匀包被硅橡胶（下载资料4），有标记、已消毒，即买即用型，不需任何处理。包被硅胶的直径及对应的动物体重见附表。如客户订做小鼠的新型号硅胶线（指硅胶直径>0.22的型号），则每次最少10根/400元，如超过30根，仍按35元/根；如客户订做大鼠的新型号硅胶线（指硅胶直径>0.28的其它型号），则每次最少10根/300元，如超过30根，仍按25元/根。A5栓线插线操作相对难于其它级别，建议预实验时，另选一个相邻的较小体重级别的型号，如大鼠体重为240-270g，除了选2634型号的硅胶线，最好再选一个对应200-240g体重的型号。另外还可以选硅胶长度为3-4mm的硅胶线，相对好操作一些。
* 大鼠线栓的标记在距头端19-20mm处，小鼠线栓的标记在距头端9-10mm处.
^# 前端20mm(大鼠线栓)或10mm(小鼠线栓)包被多聚-L-赖氨酸（包被多聚-L-赖氨酸不会改变栓线的直经）。栓线包被多聚-L-赖氨酸，可以提高MCAO模型的稳定性。

最新MCAO栓线型号/价格表，请致电13301307459或4008636007，或发邮件ysf_ok@sina.com, mcaomodel@163.com

附录：使用我们的MCAO栓线最近发表的部分SCI英文论文
（论文中的“Beijing Sunbio Biotech Co. Ltd.”既是西浓科技的前身）
1. Lin J, Zheng C, Zhang X, et al. Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke. Evidence-Based Complementary and Alternative Medicine. Volume 2015, Article ID 394926, 10 pages.
2. Lian T, Qu D, Zhao X, et al. Identification of Site-Specific Stroke Biomarker Candidates by Laser Capture Microdissection and Labeled Reference Peptide. Int. J. Mol. Sci. 2015, 16, 13427-13441.
3. Lin M, Sun W, Gong W, et al. Methylophiopogonanone A Protects against Cerebral Ischemia/Reperfusion Injury and Attenuates Blood-Brain Barrier Disruption In Vitro. PLOS ONE, April 21, 2015.
4. Zhou F, Gao S, Wang L, et al. Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model. Stem Cell Research & Therapy (2015) 6:92.
5. Wen Y, Zhang X, Dong L, et al. Acetylbritannilactone Modulates MicroRNA-155-Mediated Inflammatory Response in Ischemic Cerebral Tissues. Mol Med. 2015; 21(1): 197–209.
6. Yao H, Gao M, Ma J, et al. Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. PLoS One. 2015; 10(9): e0137211.
7. Fang L, Gao H, Zhang W, et al. Resveratrol alleviates nerve injury after cerebral ischemia and reperfusion in mice by inhibiting inflammation and apoptosis. Int J Clin Exp Med. 2015; 8(3): 3219–3226.
8. Hua K, Sheng X, Li TT, et al. The edaravone and 3-n-butylphthalide ring-opening derivative 10b effectively attenuates cerebral ischemia injury in rats. Acta Pharmacol Sin. 2015 Aug; 36(8): 917–927.
9. Lan Z, Xu X, Xu W, et al. Discovery of 3-n-butyl-2,3-dihydro-1H-isoindol-1-one as a potential anti-ischemic stroke agent. Drug Des Devel Ther. 2015; 9: 3377–3391.
10. Li X, Li C, Wei LY, et al. Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats.Neural Regen Res. 2015 Jun; 10(6): 932–937.
11. Jiang M, Li J, Peng Q, et al . Neuroprotective effects of bilobalide on cerebral ischemia and reperfusion injury are associated with inhibition of pro-inflammatory mediator production and down-regulation of JNK1/2 and p38 MAPK activation. J Neuroinflammation. 2014; 11: 167.
12. Feng R, et al. Pre-ischemic exercise alleviates oxidative damage following ischemic stroke in rats. Exp Ther Med. 2014 Oct; 8(4): 1325–1329.
13. Nie B, et al. A Statistical Parametric Mapping Toolbox Used for Voxel-Wise Analysis of FDG-PET Images of Rat Brain. PLoS One. 2014; 9(9): e108295.
14. Wang Z, et al. PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury. Evid Based Complement Alternat Med. 2014; 2014: 430757.
15. Yu S, et al. An Active Component of Achyranthes bidentataPolypeptides Provides Neuroprotection through Inhibition of Mitochondrial-Dependent Apoptotic Pathway in Cultured Neurons and in Animal Models of Cerebral Ischemia. PLoS One. 2014; 9(10): e109923.
16. Sun J, et al. Gradually Increased Training Intensity Benefits Rehabilitation Outcome after Stroke by BDNF Upregulation and Stress Suppression. Biomed Res Int. 2014; 2014: 925762.
17. Li H, et al. Isoflurane postconditioning reduces ischemia-induced nuclear factor-κB activation and interleukin 1β production to provide neuroprotection in rats and mice.
18. Zhang N, et al. Chrysophanol Inhibits NALP3 Inflammasome Activation and Ameliorates Cerebral Ischemia/Reperfusion in Mice. Mediators Inflamm. 2014; 2014: 370530.
19. Luo Y, Yang YP, Li WH, et al. Neuroprotective effects of madecassoside against focal cerebral ischemia reperfusion injury in rats. Brain Research. Available online 13 April 2014.
20. Wang ZX, Huang WQ, Zuo ZY. Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat. Journal of Neuroinflammation 2014, 11:56.
21. Sun L, Qiang R, Yang Y, et al. L-Serine Treatment May Improve Neurorestoration of Rats after Permanent Focal Cerebral Ischemia Potentially Through Improvement of Neurorepair. PLoS ONE 2014, 9(3): e93405. doi: 10.1371/journal.pone.009340.
22. Li L, et al. Glutamate transporter type 3 mediates isoflurane preconditioning-induced acute phase of neuroprotection in mice.
23. Zhu X, et al. Electroacupuncture preconditioning-induced neuroprotection may be mediated by glutamate transporter type 2.
24. Lin Y, Zhang JC, Fu J, et al. Journal of Cerebral Blood Flow & Metabolism (2013) 33, 253–262;
25. An S, Kuang YY, Shen T, et al. Brain-targeting delivery for RNAi neuroprotection against cerebral ischemia reperfusion injury. Biomaterials 34 (2013) 8949-8959.
26. Fu J, Xue R, Gu JF, et al. Neuroprotective effect of calcitriol on ischemic/reperfusion injury through the NR3A/CREB pathways in the rat hippocampus. Molecular Medicine Reports. 2013, 1708-1714.
27. Cheng FF, Lu Y, Zhong XG. Baicalin's Therapeutic Time Window of Neuroprotection during Transient Focal Cerebral Ischemia and Its Antioxidative Effects In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine, vol.2013, PP.1-11, 2013.
28. Chen XY, Sun XC, Su HX. Upregulation of myeloid cell leukemia-1 potentially modulates beclin-1-dependent autophagy in ischemic stroke in rats. BMC Neuroscience, 2013, 14:56.
29. Dong BB, Cai M, Fang ZP. Hemopexin induces neuroprotection in the rat subjected to focal cerebral ischemia. BMC Neuroscience 2013, 14:58.
30. Lan R, Xiang J, Zhang Y. PI3K/Akt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats. Evidence-Based Complementary and Alternative Medicine, Volume 2013.
31. Li JJ, Sheng WL, Feng CZ. Pyrrolidine dithiocarbamate attenuates brain Aβ increase and improves long-term neurological outcome in rats after transient focal brain ischemia. Neurobiol Dis. 2012; 45(1): 564–572.
32. Guo JY, Ding J, Yuan F. Dose-Dependent Protective Effect of Bisperoxovanadium against Acute Cerebral Ischemia in a Rat Model of Ischemia/Reperfusion Injury. Int. J. Mol. Sci. 2013, 14, 12013-12022.
33. Li LL, Zuo ZY. Isoflurane postconditioning induces neuroprotection via Akt activation and attenuation of increased mitochondrial membrane permeability. Neuroscience. 2011 December 29; 199: 44–50.
34. Li H, Yin JB, Li LL, et al. Isoflurance postconditioning reduces ischemia-induced nuclear factor-kB activation and interleukin 1β production to provide neuroprotection in rats and mice. Neurobiology of Disease. Available online 8 January 2013.
35. Zhu SP, Li Y, Lu HY, et al. Imaging the Early Cerebral Blood Flow Changes in Rat Middle Cerebral Artery Occlusion Stroke Model. 34th Annual International Conference of the IEEE EMBS San Diego, California USA, 28 August - 1 September, 2012.
36. Cheng FF, Zhong XG, Lu Y, et al. Refined Qingkailing ProtectsMCAOMice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window. Evidence-Based Complementary and Alternative Medicine. 2012, Article ID 567872, 12 pages.
37. Zhai FG, Zhang XP, Guan Y, et al. Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats. Neural Regen Res. 2012; 7(12):917-923.
38. Li LL, Zuo ZY. Glutamate transporter type 3 knockout reduces brain tolerance to focal brain ischemia in mice.Journal of Cerebral Blood Flow & Metabolism . 2011; 31:1283-1292.
39. Ding H, Yan C-Z, Shi H, Zhao Y-S, Chang S-Y, et al. Hepcidin Is Involved in Iron Regulation in the Ischemic Brain. PLoS ONE.2011, 6(9): e25324.
40. Han QQ, Li B, Feng H, et al. The promotion of cerebral ischemia recovery in rats by laminin-binding BDNF. Biomaterials. 2011; 32(22):5077-5085.
41. Xu HY, Zhang CW, Zhang CX. Effect of propofol pretreatment on apoptosis in rat brain cortex after focal cerebral ischemia and reperfusion. Neural Regen Res. 2011;6(14):1086-1090.
42. Liu K, Li Z, Wu T, et al. Role of Rho Kinase in Microvascular Damage Following Cerebral Ischemia Reperfusion in Rats. Int. J. Mol. Sci. 2011; 12: 1222-1231.
43. Yang L, Zhang BS, Yin L, et al. Tanshinone IIA Prevented Brain Iron Dyshomeostasis in Cerebral Ischemic Rats. Cell Physiol Biochem, 2011; 27:23-30.
44. Sun L, Ai J, Wang N, et al. Cerebral Ischemia Elicits Aberration in Myocardium Contractile Function and Intracellular Calcium Handling. Cell Physiol Biochem 2010; 26:421-430.
45. Zhang F, Wu Y, Jia J et al. Pre-Ischemic Treadmill Training Induces Tolerance to Brain Ischemia: Involvement of Glutamate and ERK1/2. Molecules. 2010；15： 5246-5257.
46. Wu Y, Ye XH, Guo PP, et al. Neuroprotective Effect of Lipoxin A4 Methy Ester in a Rat Model of Permanent Focal Cerebral Ischemia. J Mol Neurosci. 2010; 22:226-234.
47. Li LL, Zuo ZY. Isoflurane preconditioning improves short-term and long-term neurological outcome after focal brain ischemia in adult rats. J Neuroscience.2009; 164:497-506.
48. Xing BZ, Chen H, Zhang M. PhD. Ischemic Postconditioning Inhibits Apoptosis after Focal Cerebral Ischemia/Reperfusion Injury in the Rat. Stroke. 2008; 39:2362-2369.
49. Xing BZ, Chen H, Zhang M, et al. Ischemic post-conditioning protects brain and reduces inflammation in a rat model of focal cerebral ischemia/reperfusion. Journal of Neurochemistry. 2008; 105(5):1737-1745.