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英文名 :QuantiChrom™ α-Amylase Assay Kit
库存 :现货
供应商 :群晓科苑
保存条件 :2-8°C
规格 :48T
产品名称 :α-淀粉酶测试盒 | QuantiChrom™ α-Amylase Assay Kit
分类名称 :
酶活性测定,血液尿液化学
产品介绍 :
测定方法 比色法(595nm)定量测定α-淀粉酶活性。
产品说明 淀粉酶属于糖苷水解酶一族,其作用于α- 1 ,4 -糖苷键将淀粉分解成葡萄糖分子。 α-淀粉酶(EC 3.2.1.1)可在淀粉链上的任意位置切割,最终产生麦芽三糖、麦芽糖、葡萄糖以及来自直链淀粉和支链淀粉的“短糊精”。α-淀粉酶是哺乳动物的一种主要消化酶。人体中淀粉酶水平的增加与唾液腺创伤,及唾液腺、胰腺发炎和肾功能衰竭引起的腮腺炎有关。简单、直接、自动化的α-淀粉酶活性检测手段十分令人期待。本公司的α-淀粉酶测试盒运用α-淀粉酶裂解一种不溶性蓝色直链淀粉成可溶性有色产物。在595nm处的吸光强度与样品中淀粉酶的活性成正比。
适用范围 直接检测血清、血浆、唾液、尿液和其它生物样品中α-淀粉酶的活性。
产品特点 灵敏、准确:96孔板线性检测范围2 - 300 U/L。 方便:仅需添加单一试剂5分钟后于室温或37°C下读取吸光度。
储存条件: 将所有的试剂在2-8°C下贮存。
Description Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure.Simple, direct and automation-ready procedures for measuring α-amylase activity are very desirable. our QuantiChromTM α-amylase Assay uses an insoluble dye-coupled substrate amylose azure, which is cleaved by α-amylase into soluble colored products. The color intensity, measured at 595 nm, is proportionate to the enzyme activity in the sample.
Applications Direct assays of α-amylase activity in serum, heparinized plasma, saliva, urine and other biological samples.
Key features Sensitive and accurate. Linear detection range 2 to 300 U/L α-amylase in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at 5 min at room temperature or 37°C.
Storage conditions. Store all reagents at 2-8°C. Shelf life of at least 6 months (see expiry dates on labels).
其他 :
编号:DAMY-048,DAMY-100
测试:48T,100T
公司简介:
博世生物提供优质、快速(如2分钟)、方便的生化检测试剂盒,广泛适用于人和动物等生物样品、食品和环境监测样品中相关指标的检测。博世专注于无害、无放 射性的检测技术(如吸收光、荧光、生物化学发光法等),安全可靠,适合各类仪器(分光光度仪、微孔读数仪等)。产品具有快速、简单、高通量(可用于HTS 药物筛选)、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征。检测步骤简便,用户不需花额外的时间来作调适测试。博世生物产品主要包括:1、血液尿液化学;2、酶活性测定;3、能量代谢;4、氧化应激;5、高通量HTS试剂;6、阴阳离子检测。
北京群晓科苑生物技术有限公司
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入驻年限:12年