Quant-iT™ PicoGreen® dsDNA Assay Kit 价格_品牌:Calbiochem-丁香通官网

英文名：Quant-iT™ PicoGreen® dsDNA Assay Kit

库存：3周

供应商：研卉生物

规格：1 mL kit/200 assays

Quant-iT™ PicoGreen® dsDNA Assay Kit 优惠促销

Quant-iT™ PicoGreen® dsDNA Assay Kit
描述
通过 PicoGreen® dsDNA 定量分析，可以在含有 ssDNA、RNA 和游离核苷酸的样品中检测低至 25 pg/ml 的 dsDNA (1)。该分析在三个数量级范围内呈线性，并且具有较低的序列依赖性，因而可以准确测量多种来源的 DNA，包括基因组 DNA、病毒 DNA、小量提取 DNA 或 PCR 扩增产物。 PicoGreen® dsDNA 定量试剂 (PicoGreen® dsDNA Quantitation Reagent) 具有如下特点：

• 灵敏度比 UV 吸收读数高出几个数量级，节省了珍贵的样品（图 1）

• 在存在等摩尔量 RNA 的情况下，对 dsDNA 具有特异性（图 2）

• 易于使用-只需将染料添加到样品中，等待 5 分钟即可读取

• 适用于 96 孔和 384 孔板形式

• 与大多数荧光酶标仪、荧光计和荧光反应兼容

应用： PicoGreen® dsDNA 定量试剂 (PicoGreen® dsDNA Quantitation Reagent) 和试剂盒是 PCR 分析、样品芯片分析、DNA 损伤分析、酶活性分析、基因组 DNA 定量、复杂混合物中的 dsDNA 定量和病毒 DNA 定量的理想选择。
常用规格
Sample Type (General):

dsDNA
Platform:

Microplate Reader
Quantitation Range:

50 pg to 2 µg
Product Size:

1 mL
Shipping Condition:

Room Temperature
Regulatory Statement:

For Research Use Only. Not for use in diagnostic procedures.
参考文献：

Cell cycle synchronization of Escherichia coli using the stringent response, with fluorescence labeling assays for DNA content and replication.

作者: Ferullo DJ, Cooper DL, Moore HR, Lovett ST,
杂志: Methods (2009) 48:8-13
产品用途: (1) Flow cytometric analysis of E. coli DNA content. (2) Fluorescence microscopy analysis of E. coli size and septation (FM 4-64). (3) Measurement and visualization of newly replicated DNA in E. coli (EdU). proliferation, DNA synthesis, imaging, Click-iT Edu.
ID:: PN67515

Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain

作者: Ikeda Y, Iwakiri S, Yoshimori T,
杂志: J Pharm Biomed Anal (2009) 49:997-1002
产品用途: Assay for quantitation of host cell DNA carryover in recombinant protein products
ID:: PN67674

Factors affecting quantification of total DNA by UV spectroscopy and PicoGreen fluorescence.

作者: Holden MJ, Haynes RJ, Rabb SA, Satija N, Yang K, Blasic JR,
杂志: J Agric Food Chem (2009) 57:7221-7226
产品用途: Quantitation of DNA isolated from plant tissues (maize and soybean)
ID:: PN67675

Mitochondrial DNA is a direct target of anti-cancer anthracycline drugs.

作者: Ashley N, Poulton J,
杂志: Biochem Biophys Res Commun (2009) 378:450-455
产品用途: Flow cytometric quantification of mitochondrial DNA (mtDNA) via selective quenching of nuclear PicoGreen dye fluorescence by doxorubicin.
ID:: PN67546

The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization.

作者: He J, Mao CC, Reyes A, Sembongi H, Di Re M, Granycome C, Clippingdale AB, Fearnley IM, Harbour M, Robinson AJ, Reichelt S, Spelbrink JN, Walker JE, Holt IJ
杂志: J Cell Biol (2007) 176:141-146
ID:: PN58836