SSH cDNA library construction service

服务名称 ：SSH cDNA library construction service

提供商 ：北京安必奇生物科技有限公司

http://www.cd-genomics.com/SSH-cDNA-library-construction-service.html

Suppression subtractive hybridization (SSH) is one of the most powerful and popular PCR-based methods for isolating differentially expressed transcripts. Using a small quantity of either total RNA or poly(A)+ mRNA from each of two populations, the SSH procedure simultaneously subtracts and partially normalizes the abundance of target cDNA in the subtracted population. The clones are ligated into a vector, plated, picked, and sequenced. These genes are then available for analysis and further investigation by means of follow-on projects.

Upon completion of the subtraction, the customer will receive:

• • An Excel listing of all genes sequenced. All gene sequences belong to the customer.
• • Glycerol stocks of the forward and reverse libraries. If requested, glycerol stocks of the unsubtracted forward and reverse libraries.

With our flexible pricing, CD Genomics can begin the suppression subtractive hybridization procedure with tissue, total RNA, or poly(A)+ mRNA that you provide. Pricing is based on how much of the preparation is done by the customer, how much starting material is provided, and how many clones are sequenced.