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样本 :Serum, Plasma
适应物种 :MOUSE
检测方法 :夹心
检测限 :15.6-1000 pg/mL
库存 :10
供应商 :研卉生物
规格 :96T
Mouse IL-7 ELISA KitMouse IL-7 ELISA Kit
Catalog number: KE10060
Overview
Product name:
Mouse IL-7 ELISA Kit
Tests:
1 X 96 well plate
Sample type:
Serum, Plasma
Assay type:
Sandwich
Sensitivity:
3.6 pg/mL
Range:
15.6-1000 pg/mL
Reacted Species:
Mouse
Tested applications:
Sandwich ELISA
Recovery:
Sample Type | Average | Range |
---|---|---|
Serum | 95% | 83%-116% |
IntraAssay:
Sample | n | mean (pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | 457.2 | 24.1 | 5.3 |
2 | 20 | 124.7 | 4.8 | 3.8 |
3 | 20 | 33.0 | 1.7 | 5.3 |
InterAssay:
Sample | n | mean (pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 24 | 607.2 | 34.8 | 5.7 |
2 | 24 | 148.4 | 9.2 | 6.2 |
3 | 24 | 43.9 | 2.6 | 5.9 |
Product overview:
KE10060 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-7 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-7. The assay recognizes mouse IL-7. An antibody specific for mouse IL-7 has been pre-coated onto the microwells. The mouse IL-7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-7 is added to detect the captured mouse IL-7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm
Properties
Storage Instructions:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms:
A630026I06Rik, hlb368, Il 7, Il7, IL-7, interleukin 7, Interleukin-7
上海研卉生物科技有限公司
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入驻年限:10年