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保质期 : 一年
英文名 :GenJet™ In Vitro DNA Tranfection Reagent for PC-12 Cell
库存 : 现货 大量
供应商 : 济南美中清水湾生物科技有限公司
保存条件 : 4°C
规格 :毫升/支
Description
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 660 transfections in 24 well plates or 330 transfections in 6 well plates.
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 660 transfections in 24 well plates or 330 transfections in 6 well plates.
Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 50% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
济南思科生物科技有限公司
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入驻年限:2年