SignaGen转染试剂 for Rin Related Cells

Description
GenJet™ DNA In Vitro Tranfection Reagent for Rin is pre-optimized znd conditioned for transfecting Rin and related cells including Rin-14B, Rin-m5F, Rin-m and Rin-5F. Refer to the following optimal transfection conditions for maximal transfection efficiency on Rin related cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions Transfection Medium GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium 5 Hours After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %)

~90% DMEM with 4.5 g/L glucose, 10% FBS DMEM with 4.5 g/L glucose, 1% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes in presence of 1% FBS No 48 hours EGFP pEGFP-N3 (CMV promoter) ~85%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on Rin-5F Cells. Rin-5F cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection

Data Sheet