Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the
media selector tool.
This DMEM is modified as follows:
| With |
Without |
| • Low Glucose |
• Sodium Bicarbonate |
| • L-glutamine |
• HEPES |
| • Sodium Pyruvate |
|
| • Phenol Red |
|
The complete
formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the
original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g / L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
