Mix & Go! E.coli Transformation Kit and Buffer Set(大肠杆菌高效率转化试剂盒)

保质期 ：1年

英文名 ：Mix & Go! E.coli Transformation Kit and Buffer Set

库存 ：大量

供应商 ：简石生物

保存条件 ：4度

规格 ：20ml

HIGHLIGHTS

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• Easy 3 Step Protocol: Produce reliable chemically competent E. coli in less than 45 minutes.
• Simple 20 sec Transformation: No heat shock! Just add DNA and spread.
• High Transformation Efficiencies: Achieve 10⁸-10⁹ transformants per µg of plasmid DNA.

DESCRIPTION

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The Mix & Go! E. coli Transformation Kit and Mix & Go! E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go! method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go! bacterial transformation can be performed by adding DNA to Mix & Go! competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 10⁸-10⁹ transformants/µg plasmid DNA with most E. coli strains. Uniquely formulated reagents make it easy to generate Mix & Go! competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Mix & Go! E. coli Transformation Kit includes all buffers and ZymoBroth medium to generate 20 ml of Mix & Go! competent cells. The Mix & Go! E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Mix & Go! competent cells, and the medium (broth) is supplied by the user.