CST.9272S Akt Antibody 100 ul ( 10 western blots )

价格： ¥2972

品牌：CST

货号：9272S

产品详情

Application	Dilution	Species-Reactivity	Sensitivity	MW (kDa)	Source
W	1:1000	Human, Mouse, Rat, Hamster, Monkey,Chicken, D. melanogaster, Bovine, Pig,Guinea Pig	Endogenous	60	Rabbit
IP	1:50
IF-IC	1:200
F	1:50

Western Blotting

Western blot analysis of extracts from CHO cells, transfected with non-targeted (-) or SignalSilence^®Akt siRNA I (+) siRNA, using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. The Akt antibody confirms silencing of protein expression while the p42 MAP Kinase (Erk2) antibody was used to control for loading and specificity of Akt siRNA (A). Phospho-GSK-3β (Ser9) Antibody #9336 was used to confirm downstream pathway inhibition (B).

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® Akt siRNA I (+), using Akt Antibody #9272 and p42 MAP Kinase (Erk2) Antibody #9108. Akt antibody confirms silencing of Akt expression, while the p42 MAP kinase (Erk2) antibody is used to control for loading and specificity of Akt siRNA.

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (50 ng/ml) for the indicated times, using Phospho-Akt (Ser473) Antibody #9271 (upper) or Akt Antibody (lower).

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or LY294002-treated (green), using Akt Antibody compared to a nonspecific negative control antibody (red)

IF-IC

Confocal immunofluorescent images of C2C12 cells showing nuclear and cytoplasmic localization with Akt Antibody (left, red) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin.