EpiGnome™ Methyl-Seq Kit（全基因组甲基化测序试剂盒）

价格：询价

品牌：epicentre 品牌认证

货号：EGMK81312

产品详情

相关推荐

保质期：1年

英文名：EpiGnome™ Methyl-Seq Kit

规格：12 次反应

Epicentre的全基因组甲基化测序试剂盒，仅需50纳克基因组DNA可完成全基因组亚硫酸氢钠法测序。EpiGnome™利用独有的"post-bisulfite conversion"文库构建方法，这样能构建出高度多样化覆盖均匀的文库。
• 预制文库亚硫酸盐转化
• 高度多样性文库
• Uniform CpG, CHG, and CHH coverage
• 全步骤仅需一天
• 仅需50纳克DNA

Application:

Library Prep for Whole Genome Bisulfite Sequencing

Epicentre's methyl-seq kit produces whole genome bisulfite sequencing libraries from only 50 ng of genomic DNA. EpiGnome™ follows a unique "post-bisulfite conversion" library construction method which yields highly diverse libraries with uniform coverage.

Pre-library bisulfite conversion
Highly diverse libraries
Uniform CpG, CHG, and CHH coverage
1-day procedure
Only 50 ng into bisulfite-conversion

Figure 1 (click to enlarge). Bisulfite treated ssDNA is random primed using a polymerase able to read uracil nucleotides to synthesize DNA strands containing a specific sequence tag. The 3´-ends of the newly synthesized DNA strands are then selectively tagged with a second specific sequence tag using a patented procedure resulting in di-tagged DNA molecules with known sequence tags at its 5´ and 3´ ends. The di-tagged DNA is enriched in PCR resulting in dsDNA with the appropriate sequences required for sequencing on any Illumina platform.

Figure 2 (click to enlarge). Example of CpG methylation pattern across region of chr1 indicates high CpG methylation (red) and regions of low CpG methylation (blue) from 50 ng input of GM12878 lymphoblastoid gDNA treated with bisulfite. For the hypermetylated (CpG methylase) HeLa gDNA, majority of CpGs detected were clearly methylated. The IGV plots shown are based on forward reads for each sample.