赢润生物 VPP0042 pCI-neo 哺乳动物表达载体

品牌 ：YRBIO

货号 ：VPP0042

质粒名称：pCI-neo
出品公司：Promega
质粒用途：哺乳动物表达载体
Key Function：Constitutive Expression
Bacteria Resistance：Ampicillin
Mammalian Selection：Neo，G418
Promoter：CMV
Cloning Method：Restriction Enzyme/MCS
Constitutive or Inducible System：Constitutive

Yrbio载体库相关产品：
VPP0042，pCI-neo，900元

Description：
The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418.
(1)Strong, Constitutive Expression: The human cytomegalovirus (CMV) immediate-early enhancer/promoter region produces strong, constitutive expression. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression.
(2)Transient or Stable Expression: The neomycin phosphotransferase gene allows selection of stable transfected cells.
(3)Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximately fivefold more than the early SV40 polyadenylation signal.
(4)Convenient: Multiple cloning sites exist for easy insertion of cDNA.
(5)Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication.