保质期 :半年
英文名 ://
库存 :大量
供应商 :北京普利莱
CAS号 ://
保存条件 :4ºC保存
规格 :50次/105次
描述:甘油是甘油三酯的水解产物。与游离脂肪酸一样,甘油含量是甘油三酯水解反应的可靠检测指标,但检测更加方便。本试剂盒采用优化步骤,能够检测实体组织、细胞中的甘油含量,线性范围为10-1200µmol/L。
参考文献:
1. Trinder, P. (1969). Ann. Clin. Biochem. 6: 24 – 27.
2. Barham D and Trinder P. (1972). Analyst 97: 142 – 145
原理:在ATP存在下甘油被甘油激酶磷酸化为3-磷酸甘油,再被甘油磷酸氧化酶氧化产生过氧化氢;在过氧化物酶作用下生色底物,其光密度值与甘油浓度成正比。
适用范围:测定实体组织、细胞中的甘油浓度。
组成 (105次微板测定或30次1ml比色杯测定):
(1) 裂解液 100 ml
(2) R1试剂 16 ml
(3) R2试剂 4 ml
(4) 4 mmol/L甘油标准品1 ml
以上组分均4 ºC储存,6个月有效。
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