AssayPro—Human tPA Chromogenic Activity Assay Kit（组织纤溶酶原激活剂tPA发色底物法活性试剂盒）

供应商 ：AssayPro

检测限 ：0.03 IU/ml

检测方法 ：发色底物法

应用 ：血浆、细胞培养上清液

样本 ：20 μl

规格 ：96 wells

Introduction
Tissue-type plasminogen activator (tPA) is a 68-kDa serine protease that converts the zymogen plasminogen into the active serine protease plasmin, which digests fibrin and induces the dissolution of fibrin clots (1). tPA is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo (2). A high level of tPA is a good prognostic marker for breast cancer (3). tPA may minimize the formation of metastasis by preventing tumor cell adherence at sites of trauma (4). On the other hand, gastrointestinal cancer is accompanied by a decrease in tPA (5).

Principle of Assay
The AssaySense Human tPA Chromogenic Activity Kit is developed to determine human tPA activity in plasma and cell culture supernatants. The assay measures the ability of tPA to activate the plasminogen to plasmin in coupled or indirect assays that contain tPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the tPA enzymatic activity.