AssayPro—Human Total tPA ELISA Kit（总组织纤溶酶原激活剂tPA酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：0.1 ng/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液、组织提取液

样本 ：50 μl

规格 ：96 wells

Introduction
Tissue-type plasminogen activator (tPA) is a serine protease that converts the zymogen plasminogen into the active serine protease plasmin, the primary enzyme responsible for the removal of fibrin deposits (1). tPA is a 68 kDa glycoprotein that is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo (2). A high level of tPA is a good prognostic marker for breast cancer (3 - 4). tPA may minimize the formation of metastasis by preventing tumor cell adherence at sites of trauma (5). On the other hand, gastrointestinal cancer is accompanied by a decrease in tPA (6).

Principle of the Assay
The AssayMax Human Total tPA ELISA kit is designed for detection of human tPA in plasma, serum, milk, cell culture supernatants, and tissue extract. This assay employs a quantitative sandwich enzyme immunoassay technique that measures total tPA in less than 4 hours. A polyclonal antibody specific for tPA has been pre-coated onto a microplate. tPA in standards and samples is sandwiched by the immobilized antibody and a biotinylated antibody specific for tPA, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.