AssayPro—Human Tissue Factor Chromogenic Activity Kit (组织因子TF发色底物法活性试剂盒)-两步法

供应商 ：AssayPro

检测限 ：15 pM

检测方法 ：发色底物法

应用 ：血浆、组织、细胞培养上清液

样本 ：10 μl

规格 ：96 wells

Introduction
跨膜糖蛋白组织因子（TF）是生理性凝血过程的启动物，TF可结合并激活VII，TF-VIIa复合物又可进一步激活因子IX和因子X，导致凝血酶的生成。很多病理条件下，如革兰氏阴性败血症、急性冠脉综合症等都可诱导TF的表达，后者与这些疾病过程中危及生命的血栓形成有关，败血症中血管内TF表达可导致弥散性血管内凝血。TF还在血管发生、转移、及肿瘤相关的血管生成方面发挥重要作用。

Principle of Assay
The AssaySense Human Tissue Factor Chromogenic Activity Assay Kit is developed to determine human TF chromogenic activity in plasma, tissue, and cell culture supernatants. The assay measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.