AssayPro—Human Transferrin ELISA Kit（人转铁蛋白TRF酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：0.09 μg/ml

检测方法 ：ELISA

应用 ：血清、血浆

样本 ：25 μl

规格 ：96 wells

Introduction
Transferrin is a plasma protein that transports iron through the blood to the liver, spleen, and bone marrow. Low transferrin levels in plasma could associate with anemia (1) and chronic liver disease (2). On the other hand, a high plasma transferrin level could indicate iron deficiency anemia (3).

Principle of the Assay
The AssayMax Human Transferrin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human transferrin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human transferrin in less than 2 hours. A polyclonal antibody specific for human transferrin has been pre-coated onto a 96-well microplate with removable strips. Transferrin in standards and samples is competed with a biotinylated transferrin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.