Human Protein S (Total) ELISA Kit（人蛋白S酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：0.25 μg/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液

样本 ：25 μl

规格 ：96 wells

Introduction
Protein S is a single-chain vitamin K-dependent plasma glycoprotein consisting of 635 amino acids with a molecular weight of about 70 kDa (1 - 3). It functions as a cofactor for the anticoagulant activated protein C to inhibit blood coagulation. In plasma, it is both in a free cofactor active form and in an inactive form complexed with C4b-binding protein (C4BP) (4). The complex acts as a bridge between coagulation and inflammation due to the involvement of C4BP in regulating complement activation. Hereditary protein S deficiency causes recurrent venous thrombosis (5). Acquired protein S deficiency is associated with nephrotic syndrome, disseminated intravascular coagulation, liver disease and the use of oral anticoagulants, leading to increased thrombotic risk (6).

Principle of the Assay
The AssayMax Human Protein S ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human protein S in plasma, serum and cell culture samples. This assay employs a quantitative competitive enzyme immunoassay technique that measures human protein S in less than 3 hours. A polyclonal antibody specific for human protein S has been pre-coated onto a 96-well microplate with removable strips. Protein S in standards and samples is competed by a biotinylated protein S sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.