AssayPro—Human IL-1β ELISA Kit（人白介素1β酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：3.9 pg/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液

样本 ：50 μl

规格 ：96 wells

Introduction
Interleukin-1beta (IL-1beta) has a wide spectrum of inflammatory, metabolic, haemopoietic, and immunological properties (1). IL-1beta plays a significant role in hippocampal synaptic function (2) and is a potential genetic marker as an indicator of gastric cancer risk (3). High plasma levels of interleukin-1beta (IL-1beta) is associated with rheumatoid and osteoarthritic joint disease (4), infectious gastroenteritis (5), neurodegeneration (6), and breast cancer (7). High gingival crevicular fluid levels of interleukin-1beta is related to type 2 diabetes (8).

Principle of the Assay
The AssayMax Human IL-1beta ELISA kit is designed for detection of human IL-1beta in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures IL-1beta in less than 5 hours. A murine monoclonal antibody specific for IL-1beta has been pre-coated onto a microplate. IL-1beta in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for IL-1beta, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.