AssayPro—Human IgG ELISA Kit（免疫球蛋白G酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：0.6 ng/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液、唾液、尿液、牛奶

样本 ：50 μl

规格 ：96 wells

Introduction
Human Immunoglobulin G (IgG), the most abundant antibody in serum, constitutes 75% of serum immunoglobulins. IgG is synthesized and secreted by plasma B cells and contains two heavy chains and two light chains. IgG has four subclasses IgG1, IgG2, IgG3, and IgG4 and is involved in the secondary immune response. As it is the only isotype that can pass through the human placenta, maternal IgG provides the defense against infection for the first few weeks of a neonate (1). IgG has been shown to treat autoimmune disease, induce apoptosis, and stimulate complement attenuation (3-4). Elevated IgG is observed in viral hepatitis, autoimmune hepatitis, and cirrhosis (5).

Principle of the Assay
The AssayMax Human IgG ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of human IgG in plasma, serum, saliva, urine, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IgG in less than 4 hours. A polyclonal antibody specific for human IgG has been pre-coated onto a 96-well microplate with removable strips. IgG in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for IgG, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.