AssayPro—Human Heat shock proteins 60 ELISA Kit（热休克蛋白HSP60酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：2 ng/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液、组织提取液

样本 ：50 μl

规格 ：96 wells

Introduction
Heat shock protein of 60 kDa (HSP60) is a mitochondrial chaperonin involved in folding, assembly, and transport of newly imported protein from cytoplasm into mitochondria in an ATP-mediated reaction (1-3). Human HSP60 contains 573 amino acids, is related to the bacteria groEL protein, and located in the mitochondria and cytoplasm, the cell surface, the extracellular space, and the peripheral blood (4-5). Under dehydration conditions, the cytoplasmic HSP60 is quickly imported into the mitochondria by cytoplasmic HSP70 (6). Extracellular HSP60 mediates apoptosis via Toll-like receptors in heart failure (7). HSP60 plays a role in myelinogenesis and neurodegeneration: Its defect can cause neurodegenerative pathologies (8). It has been suggested that HSP60 is linked to diabetes, stress response, cancer and immunological disorders. Since it participates in cell signaling and key pathways, and is actively secreted by human tumor cells, HSP60 could be used for cancer diagnosis and therapy (9).

Principle of the Assay
The AssayMax Human HSP60 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human HSP60 in cell culture lysates, plasma, serum, and tissue samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human HSP60 in less than 5 hours. A polyclonal antibody specific for human HSP60 has been pre-coated onto a 96-well microplate with removable strips. HSP60 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for HSP60, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.