AssayPro—Swine Haptoglobulin ELISA Kit（猪结合珠蛋白Hpt酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：1.8 ng/ml

检测方法 ：ELISA

应用 ：血浆、细胞培养上清液、尿液

样本 ：50 μl

规格 ：96 wells

Introduction
Haptoglobulin (Hpt) is a plasma protein with hemoglobin-binding capacity and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis (1). High haptoglobulin level in plasma has been associated with an increased cardiovascular risk in obese men (2), inflammation (3), atherosclerosis (4), and systemic sclerosis (5).

Principle of the Assay
The AssayMax Swine Haptoglobulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of swine haptoglobulin in plasma, urine, and cell culture supernatant samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine haptoglobulin in less than 4 hours. A polyclonal antibody specific for swine haptoglobulin has been pre-coated onto a 96-well microplate with removable strips. Swine haptoglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for swine haptoglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.