AssayPro—Bovine Haptoglobulin ELISA Kit（牛结合珠蛋白Hpt酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：3.7 ng/ml

检测方法 ：ELISA

应用 ：尿液、细胞培养上清液

样本 ：50 μl

规格 ：96 wells

Introduction
Haptoglobulin (Hpt) is a plasma protein with hemoglobin-binding capacity, and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis (1). High haptoglobulin level in plasma was associated with an increased cardiovascular risk in obese men (2), inflammation (3), atherosclerosis (4), and systemic sclerosis (5).

Principal of the Assay
The AssayMax Bovine Haptoglobulin ELISA kit is designed for detection of Haptoglobulin in bovine urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures haptoglobulin in less than 4 hours. A polyclonal antibody specific for haptoglobulin has been pre-coated onto a microplate. Haptoglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for haptoglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.