AssayPro—Human GC-Globulin ELISA Kit（维生素D结合蛋白ELISA）

库存 ：大量

供应商 ：AssayPro

检测限 ：0.09 μg/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液、唾液、牛奶

样本 ：25 μl

规格 ：96 wells

Introduction
Gc-Globulin or Vitamin D-binding protein is a multifunctional plasma protein with functions in the transport of Vitamin D metabolites, control of bone development, binding of fatty acids, sequestration of actin and a range of less-defined roles in modulating immune and inflammatory responses (1). The Gc-globulin levels on healthy individuals range from 176-623mg/L with no age dependency (2). A low serum level (< 100 mg/L) of the actin-scavenger Gc-globulin is a prognostic marker of non-survival in fulminant hepatic failure (FHF) (3), trauma, and sepsis (4). Low Gc-globulin plasma or serum levels are also linked to osteoporosis, Graves' disease, Hashimoto's thyroiditis, diabetes, COPD, AIDS, multiple sclerosis, sarcoidosis, and rheumatic fever (5).

Principle of the Assay
The AssayMax Human Gc-Globulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Gc-globulin in plasma, serum, saliva, milk, and cell culture supernatants. This assay employs a quantitative competitive enzyme immunoassay technique that measures human Gc-globulin in less than 3 hours. A polyclonal antibody specific for human Gc-globulin has been pre-coated onto a 96-well microplate with removable strips. Gc-globulin in standards and samples is competed with a biotinylated Gc-globulin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.