AssayPro—Human Fibronectin ELISA Kit（人纤维结合蛋白Fn酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：40 ng/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液

样本 ：25 μl

规格 ：96 wells

Introduction
Fibronectin (FN) is a major component of the extracellular matrix and blood plasma, and is a specific ligand for several integrin adhesion receptors (1). FN plays an important role not only in cell adhesion (2) and wound healing (3), but also in embryogenesis (4) and hematopoiesis (5). FN is over-expressed in cardiovascular disease states such as atherosclerosis (6) and myocardial infarction (7). Reduced levels of FN have been reported in patients with Disseminated Intravascular Coagulation (DIC) and low concentrations appear to correlate with a poor prognosis (8).

Principal of the Assay
The AssayMax Human Fibronectin ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative competitive sandwich enzyme immunoassay technique that measures human plasma, serum and cell culture supernatant FN in less than 3 hours. A murine antibody specific for FN has been pre-coated onto a 96-well microplate with removable strips. FN in standards and samples is competed by a biotinylated FN sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.