AssayPro—Rat Fibrinogen ELISA Kit（大鼠纤维蛋白原FBG酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：3 ng/ml

检测方法 ：ELISA

应用 ：细胞培养上清液、尿液

样本 ：50 μl

规格 ：96 wells

Introduction
Fibrinogen (FBG) is a homodimer of molecular mass 340 kDa, made up of two sets of α, β, and ɣ polypeptide chains, and synthesized in the parenchymal cell of the hepatocyte and in the megakaryocyte (1). FBG plays a major role in coagulation, and both elevated and decreased levels have clinical significance. Upon cleavage by thrombin in the initial stages of coagulation activation, FBG self-assembles to yield a fibrin clot matrix that subsequently is cross-linked by factor XIIIa to form an insoluble network. FBG also binds to the platelet glycoprotein IIbIIIa receptor to form bridges between platelets, thus facilitating aggregation (2). Elevated plasma FBG has been identified as an independent risk factor for coronary atherosclerosis and ischemic heart disease (3, 4). Individuals with congenital absence of FBG, termed afibrinogenemia, have prolonged bleeding times.

Principle of the Assay
The AssayMax Rat Fibrinogen ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of rat FBG in cell culture media and urine samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rat FBG in 4 hours. A polyclonal antibody specific for rat FBG has been pre-coated onto a 96-well microplate with removable strips. FBG in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for rat FBG, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.