AssayPro—Human Factor X(FX) ELISA Kit

库存 ：大量

供应商 ：AssayPro

检测限 ：1.5 ng/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液、尿液、唾液、牛奶

样本 ：50 μl

规格 ：96 wells

Introduction
Factor X (FX) is a plasma serine protease zymogen involved in the blood coagulation cascade. FX is purified from plasma as a two-chain protein consisting of a 45 kDa heavy chain and a 17 kDa light chain. The FX heavy chain is cleaved during coagulation by several different proteases including the intrinsic Xase complex, the FX-activating enzyme from Russell’s viper venom (RVV) and trypsin, and also by extrinsic (tissue factor/factor VIIa) pathway to give an active enzyme FXa. FXa as the activator of prothrombin occupies a central position linking the two blood coagulation pathways (1-4).

Principle of the Assay
The AssayMax Human Factor X ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human factor X in plasma, serum, milk, urine, saliva, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FX in less than 4 hours. A monoclonal antibody specific for FX has been pre-coated onto a 96-well microplate with removable strips. FX in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for FX, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.