AssayPro—Human Complement C7 ELISA Kit（补体C7酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：0.6 ng/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液、尿液、唾液、牛奶

样本 ：50 μl

规格 ：96 wells

Introduction
Human Complement Component 7 (C7) is a single-chain glycoprotein consisting of 821 amino acid residues with a molecular weight of about 97 kDa. The amino-terminal of C7 has 23 - 30% homology with Complements C8 and C9 (1). C7 is one of five complement proteins (C5b, C6, C7, C8, and C9) that assemble on pathogen membranes to form the lethal membrane attack complex. It serves as a membrane anchor for the C5b - 7 complex. C7 deficiency exhibits an increased susceptibility to Neisseria meningitidis infections and recurrent meningococcal disease (2 - 3). Plasma C7 is also associated with asthma exacerbation (4), age-related macular degeneration (5), and multiple sclerosis (6).

Principle of the Assay
The AssayMax Human Complement C7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of C7 in human plasma, serum, urine, milk, saliva, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C7 in less than 4 hours. A polyclonal antibody specific for C7 has been pre-coated onto a microplate. C7 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C7, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.